Calbindin D9K Antibody (D-5): sc-74462

Calbindin D9K Antibody (D-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Calbindin D9K of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Calbindin D9K antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Calbindin D9K plays a crucial role in calcium homeostasis, acting as a calcium-binding protein that helps regulate intracellular calcium levels, which is vital for numerous cellular processes, including muscle contraction, neurotransmitter release, and cellular signaling. Calbindin D9K presence in tissues such as cartilage, bone, and specific epithelial cells underscores its importance in maintaining calcium balance and supporting cellular functions. The gene encoding human Calbindin D9K is located on the X chromosome and consists of three exons, with expression being tightly regulated by various factors, including hormonal influences. Calbindin D9K interaction with calcium ions is facilitated by EF-hand motifs, which are essential for function in calcium signaling pathways. Understanding Calbindin D9K role in these processes can provide insights into various physiological and pathological conditions, making Calbindin D9K monoclonal antibody (D-5) an invaluable tool for researchers studying calcium-related functions in human tissues.

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Calbindin D9K Antibody (D-5) References:

1. Calbindin-D9k. A vitamin-D-dependent, calcium-binding protein in mineralized tissues.  |  Balmain, N. 1991. Clin Orthop Relat Res. 265-76. PMID: 2009668
2. Calcium-binding proteins of the EF-type.  |  Heizmann, CW. 1988. J Cardiovasc Pharmacol. 12 Suppl 5: S30-7. PMID: 2469875
3. Gestational changes in calbindin-D9k in rat uterus, yolk sac, and placenta: implications for maternal-fetal calcium transport and uterine muscle function.  |  Mathieu, CL., et al. 1989. Proc Natl Acad Sci U S A. 86: 3433-7. PMID: 2717621
4. Immunochemical localization of vitamin D-dependent calcium-binding protein in mouse placenta and yolk sac.  |  Bruns, ME., et al. 1985. Anat Rec. 213: 514-7, 532-5. PMID: 4083532
5. Placental calcium binding protein in rats. Apparent identity with vitamin D-dependent calcium binding protein from rat intestine.  |  Bruns, ME., et al. 1978. J Biol Chem. 253: 3186-90. PMID: 417082
6. Vitamin D-dependent calcium-binding protein. Changes during gestation, prenatal and postnatal development in rats.  |  Delorme, AC., et al. 1979. J Dev Physiol. 1: 181-94. PMID: 551109
7. C cell activity during the prenatal and postnatal periods in the rat.  |  Garel, JM., et al. 1981. Endocrinology. 109: 1573-7. PMID: 7028461
8. Biochemical characterization of mouse vitamin D-dependent calcium-binding protein. Evidence for its presence in embryonic life.  |  Delorme, AC., et al. 1982. Biochem J. 205: 49-57. PMID: 7126183
9. Intestinal and placental calcium-binding proteins in vitamin D-deprived or -supplemented rats.  |  Marche, P., et al. 1978. Life Sci. 23: 2555-61. PMID: 739838
10. The human calbindin-D9k gene. Complete structure and implications on steroid hormone regulation.  |  Jeung, EB., et al. 1994. J Mol Biol. 235: 1231-8. PMID: 8308886
11. Control of vitamin D-dependent calcium-binding protein in rat intestine by growth and fasting.  |  Bruns, ME., et al. 1977. J Biol Chem. 252: 4145-50. PMID: 863920
12. Genetic regulation of placental function: a quantitative in situ hybridization study of calcium binding protein (calbindin-D9k) and calcium ATPase mRNAs in sheep placenta.  |  Morgan, G., et al. 1997. Placenta. 18: 211-8. PMID: 9089784

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calbindin D9K Antibody (D-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Calbindin D9K Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calbindin D9K Antibody (D-5) Specifications

Alternate Product Name: S100 calcium binding protein G (S100G)

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-60

Epitope Target: Calbindin D9K

Epitope Species: human

Product Reactivity/Detection: Calbindin D9K

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Calbindin D9K Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin