Date published: 2025-9-8

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Anticorps C3 (B-9): sc-28294

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Fiches techniques
  • L'anticorps C3 B-9 est un monoclonal IgG1 κ L'Anticorps C3, cité dans 60 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 541-840 de C3 precursor d'origine human
  • L'Anticorps C3 (B-9) est recommandé pour la détection de C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
  • Anti-L'Anticorps C3 (B-9) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de C3 (B-9): sc-28294.
  • m-IgG Fc BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps C3 (B-9) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps C3 (B-9) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

L'anticorps C3 (B-9) est un anticorps monoclonal de chaîne légère kappa IgG1 de souris dirigé contre les acides aminés 541-840 de la protéine humaine du composant 3 (C3). Le C3, également connu sous le nom de CPAMD1 ou de protéine stimulant l'acylation (ASP), est un composant central du système du complément, jouant un rôle crucial dans l'immunité innée. Synthétisé principalement dans le foie, le C3 est sécrété dans la circulation sanguine et existe dans l'espace extracellulaire, où il circule sous forme de précurseur inactif. Lors de l'activation, le C3 est clivé en fragments C3a et C3b; le C3a fonctionne comme une anaphylatoxine médiant les réponses inflammatoires, tandis que le C3b se lie aux surfaces des pathogènes, favorisant l'opsonisation et la phagocytose par les cellules immunitaires. La localisation extracellulaire du C3 est essentielle, car le C3 permet une interaction immédiate avec les pathogènes envahissants et l'activation de la cascade du complément à la surface des cellules. Le dérèglement de l'activation du C3 peut entraîner des troubles auto-immuns et une sensibilité accrue aux infections. L'anticorps C3 (B-9) reconnaît le précurseur C3, l'anaphylatoxine C3a, la chaîne C3 α, la chaîne C3 β et la chaîne C3b α′ de la souris, du rat et de l'homme, et est recommandé pour une utilisation en western blotting (WB), immunoprécipitation (IP), immunofluorescence (IF), immunohistochimie avec des sections incluses en paraffine (IHCP), et enzyme-linked immunosorbent assay (ELISA). L'anticorps monoclonal C3 (B-9) est un outil précieux pour étudier les voies d'activation du complément et leurs implications dans les réponses immunitaires chez de nombreuses espèces.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Anticorps C3 (B-9) Références:

  1. Modulation de l'activité du C3a: internalisation du récepteur C3a humain et son inhibition par le C5a.  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
  2. La phosphorylation des récepteurs d'anaphylatoxines C3aR et C5aR induite par un ligand est médiée par des 'récepteurs kinases couplés aux protéines G'.  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
  3. Un rôle pour le récepteur anaphylatoxine C3a dans la phase effectrice de l'asthme.  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
  4. Composant C3 du complément humain: séquence codante de l'ADNc et structure primaire dérivée.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
  5. L'activation de la protéine kinase activée par les mitogènes nécessite deux signaux provenant du récepteur de l'anaphylatoxine humaine C5a.  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
  6. Expression médiée par le TNF-alpha du récepteur de l'anaphylatoxine C5a sur les neurones dans la méningo-encéphalite expérimentale à Listeria.  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps C3 (B-9)

sc-28294
200 µg/ml
$316.00

C3 (B-9): m-IgG Fc BP-HRP Kit

sc-528410
200 µg Ab; 10 µg BP
$354.00

C3 (B-9): m-IgGκ BP-HRP Kit

sc-520784
200 µg Ab, 40 µg BP
$354.00

Anticorps C3 (B-9) AC

sc-28294 AC
500 µg/ml, 25% agarose
$416.00

Anticorps C3 (B-9) HRP

sc-28294 HRP
200 µg/ml
$316.00

Anticorps C3 (B-9) FITC

sc-28294 FITC
200 µg/ml
$330.00

Anticorps C3 (B-9) PE

sc-28294 PE
200 µg/ml
$343.00

Anticorps C3 (B-9) Alexa Fluor® 488

sc-28294 AF488
200 µg/ml
$357.00

Anticorps C3 (B-9) Alexa Fluor® 546

sc-28294 AF546
200 µg/ml
$357.00

Anticorps C3 (B-9) Alexa Fluor® 594

sc-28294 AF594
200 µg/ml
$357.00

Anticorps C3 (B-9) Alexa Fluor® 647

sc-28294 AF647
200 µg/ml
$357.00

Anticorps C3 (B-9) Alexa Fluor® 680

sc-28294 AF680
200 µg/ml
$357.00

Anticorps C3 (B-9) Alexa Fluor® 790

sc-28294 AF790
200 µg/ml
$357.00

Hello-Which antibody pairs would you recommend to develop a C3 sandwich ELISA

Posée par: Aaron Stoker
Thank you for your question. We have not validated our antibodies for sandwich ELISA.
Répondue par: BlakeJ
Date de publication: 2024-09-20

hello,i see C3 antibody (B-9): sc-28294 is a mouse monoclonal antibody,how can it detect c3a anaphylatoxin and C3b α′ chain at the same time,if i want to use it in IHC,how can i distinguish it is C3b,C3a or c3?

Posée par: sophia
Thank you for your question. C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you would like to detect the specific protein, please select the other antibody. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Répondue par: Sen Li
Date de publication: 2023-12-25

hello,can C3 Antibody (B-9): sc-28294 be used to dectect C3a anaphylatoxin in mouse.If so,did you have any such successful examples (western images) to shown to me ?

Posée par: sophia0314
Thank you for your question. Yes, C3 Antibody (B-9): sc-28294 is recommended for detection of C3a anaphylatoxin in mouse sample by WB, IP, IF, IHC(P) and ELISA. We don't have an in-house data for detection of C3a anaphylatoxin in mouse sample by WB at this moment, but please refer to the citations for this product.
Répondue par: Technical Service SH
Date de publication: 2023-03-16

Can I use non-transfected HEK-293T as a negative control? In your legend of Near-infrared western blot analysis of C3 expression said 293T has C3 band. But same time in your "sc-28294 HRP. Direct western blot analysis" legend said 293T has no band.

Posée par: Woosung
Thank you for your question. Our Control 293T Lysate: sc-117752 is derived from non-transfected 293T cells and suitable for use as a negative control with our 293T Transfected Lysates. Our Control 293T Lysate goes through the same transfection process as our gene-specific transfected lysates, except without transfecting any cDNA into the cells. The Control 293T Lysate is also used at a lower concentration than regular cell lines for Western blotting. That said, it does appear that HEK293 does not have significant RNA expression of C3 per the Human Protein Atlas. We will review of the Near-infrared Western blot data showing a signal in HEK293T cell lysate, and if it can not be supported we will remove it.
Répondue par: Technical Support
Date de publication: 2021-07-12

Has anyone used this AB for IHC in rat brain tissue? 

Posée par: Anonyme
Thank you for your question. We do not have publications specifically in rat brain tissue, but we have many publication in other rat tissues such as kidney, intesting and heart. We do have publications in mouse brain samples, so I would expect it to work perfectly fine in rat brain as well.
Répondue par: Tech Service
Date de publication: 2021-06-01

Hi - I see that this antibody can detect C3 (which has a molecular weight of 150kDa), but that it is also recommended to detect C3a and C3b. How do I specifically probe for C3a and C3b separately on a western blot? Do I add in something to break C3 down?

Posée par: C3question
Thank you for your question. At this time, we are not able to provide any specific inductions or treatments for samples to increase expression of the different C3 subunits. This is a research-based question, and we would recommend you consult with the primary literature. There are many product citations using this antibody for Western blotting applications, so it may be useful to start by reviewing the citations on our website for this antibody.
Répondue par: Technical Support
Date de publication: 2019-07-12

Has this antibody specificity been validated? If so, can you include the link to evidence/publication? Thank you.

Posée par: Shandiz
Thank you for your question. This antibody has many data images published on this product page, all showing validation. It also has many citations showing it’s use in publications. You can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp ?_requestid=6883845
Répondue par: Technical Support
Date de publication: 2019-05-29

Under what conditions have you demonstrated a positive C3 signal in human paraffin samples? I have tried numerous epitope retrieval protocols and seen nothing. I'm disappointed that you discontinued the goat polyclonal V-20 in favor of this product.

Posée par: squareshot
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research! We use a 1:50 dilution with this antibody on paraffin sections, after a antigen retrieval in sodium citrate with heat. Please contact our Technical Service Department by phone, e-mail or live chat. We are more than happy to help you troubleshoot the antibody. Also, we do warranty our antibodies for one year from the date of purchase, so if we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Répondue par: Tech Service
Date de publication: 2017-04-12
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Rated 5 de 5 de par Works well4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked well.
Date de publication: 2018-08-01
Rated 1 de 5 de par Does not detect human C3 in paraffin tissuesI tried using this antibody to detect human C3 in paraffin-embedded tissues, using three different antigen retrieval techniques, and saw nothing. I also tried using the unconjugated antibody followed by an anti-mouse secondary, saw nothing. On the other hand, the discontinued goat polyclonal worked.
Date de publication: 2017-05-08
Rated 5 de 5 de par Great for all manner of C3 detection.This is a proven winner in C3 detection. It has broad range or reactivity and great specificity. It will provide you the results and signal you want in all standard applications. It has been published with more than 10 times.
Date de publication: 2017-01-05
Rated 5 de 5 de par Superb!!!Great western if and ihc results; strong signal and low background
Date de publication: 2017-01-05
Rated 4 de 5 de par IHC-Psome macrophages were positive for C3 by this antobody in IHC-P method
Date de publication: 2016-12-30
Rated 5 de 5 de par Great in multiple applicationsThis antibody works very well in Western Blot, IF and IHC(P)
Date de publication: 2016-12-23
Rated 5 de 5 de par I used it to detect mouse complement C3bI used it to detect mouse complement C3b with western blot. I should say that it is a wonderful product which gives a clear and concise band without obvious mixed band. I do have bought many of your product (mainly antibodies), they all works well, and the price is acceptable. You helped me a lot in my experiments, and I will keep on using your product.
Date de publication: 2015-10-15
Rated 5 de 5 de par Great stain in mouse intestinal tissuesGreat stain in mouse intestinal tissues.
Date de publication: 2015-08-06
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Anticorps C3 (B-9) est évalué 4.7 de 5 de 16.
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