C23 (Nucleolin) Antibody (D-6): sc-17826

C23 Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects C23 of human and monkey origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. C23 (D-6) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C23, also known as nucleolin (NCL), is a crucial eukaryotic nucleolar phosphoprotein playing a significant role in ribosome synthesis and maturation. C23 is primarily located in dense fibrillar regions of the nucleolus, containing four RNA binding domains that facilitate interaction with pre-ribosomal RNA during ribosome biogenesis. This localization allows C23 to influence RNA processing, ribosomal gene transcription, and nucleolar targeting of ribosomal components, ensuring efficient ribosome assembly and function. C23 interacts with various proteins, including nucleolar protein B23, while phosphorylation by Cdc2 and casein kinase II leads to translocation from nucleolus to cytoplasm, highlighting dynamic roles in cellular processes. C23 interaction with human telomerase reverse transcriptase subunit (TERT) is particularly noteworthy, being essential for nucleolar localization of TERT, linking C23 to critical pathways in cellular aging and cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-C23 (Nucleolin) Antibody (D-6) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-C23 (Nucleolin) Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

C23 (Nucleolin) Antibody (D-6) Specifications

Alternate Product Name: Nucleolin

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 271-520

Epitope Target: C23

Epitope Species: human

Product Reactivity/Detection: C23

Species Reactivity/Detection: human, monkey

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for C23 (Nucleolin) Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred C23 antibody is C23 Antibody (MS-3): sc-8031.