BAZ2B Lentiviral Activation Particles (m): sc-436575-LAC
- Target species: mouse
- 200 µl of transduction-ready, high-titer CRISPR/dCas9 Lentiviral Activation Particles
- BAZ2B Lentiviral Activation Particles (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically and efficiently upregulate gene expression via lentiviral transduction of cells
- BAZ2B Lentiviral Activation Particles (m) contain the following SAM Activation elements: a deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, an MS2-p65-HSF1 fusion protein and a target-specific 20 nt guide RNA. They also contain the blasticidin, hygromycin and puromycin resistance genes
- Upon transduction, the SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by BAZ2B Lentiviral Activation Plasmid (m) and BAZ2B Lentiviral Activation Plasmid (m2) target distinct regulatory regions of the Baz2b promoter. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAZ2B Lentiviral Activation Particles (m) | sc-436575-LAC | 200 µl | $455.00 |
BAZ2B (bromodomain adjacent to zinc finger domain protein 2B) is a nuclear chromatin-associated factor that recognizes acetylated histones via its bromodomain and contributes to regulation of transcriptional programs through epigenetic mechanisms. It is linked to ATP-dependent chromatin remodeling and can influence gene expression states involved in cell identity, proliferation, and differentiation. In mouse systems, Baz2b has been used to study how chromatin “reader” proteins shape developmental and tissue-specific transcriptional networks and how their dysregulation perturbs genome regulation. Altered regulation of bromodomain-containing chromatin factors is broadly relevant to cancer biology and neurodevelopmental phenotypes, making Baz2b a useful node for pathway-level interrogation of chromatin-driven gene control.
BAZ2B Lentiviral Activation Particles (m) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient Baz2b upregulation across a broader range of human cell types.
BAZ2B Lentiviral Activation Particles (m) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the Baz2b transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous BAZ2B expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native Baz2b genomic locus and regulatory architecture.
The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.