ASPP1 CRISPR/Cas9 KO Plasmid (m): sc-423473
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASPP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ASPP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ASPP1 Antibody (LX011): sc-53903
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASPP1 CRISPR/Cas9 KO Plasmid (m) | sc-423473 | 20 µg | $397.00 |
Overview
Ppp1r13b encodes ASPP1, an apoptosis-stimulating protein that binds the DNA-binding domain of p53 family transcription factors and biases their activity toward pro-apoptotic target genes. Through modulation of p53/p63/p73-dependent transcription, ASPP1 contributes to stress-induced apoptosis, cell-cycle control, and maintenance of genomic integrity. ASPP1 function intersects with DNA damage response signaling and transcriptional co-regulator networks that shape cell fate decisions. Altered regulation of this axis is relevant to tumor biology and other conditions where defective apoptosis and checkpoint control contribute to disease-associated cellular phenotypes.
ASPP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppp1r13b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppp1r13b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppp1r13b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ASPP1 protein expression.
This CRISPR knockout system enables efficient generation of Ppp1r13b-deficient cell models for investigation of ASPP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppp1r13b exon(s) critical for ASPP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppp1r13b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ASPP1 CRISPR/Cas9 KO Plasmid (m) and ASPP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppp1r13b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ASPP1 HDR Plasmid (m) and ASPP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppp1r13b homology arms to support homology-directed repair at defined Ppp1r13b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.