ASC/TMS1/PYCARD Antibody (D-3): sc-514559

ASC Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody specifically engineered to recognize the apoptosis-associated speck-like protein containing a CARD (ASC), also known as TMS1 or PYCARD. ASC monoclonal antibody (D-3) targets an epitope located between amino acids 170-195 at the C-terminus of human ASC, ensuring effective detection of ASC proteins in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ASC is a pivotal 195 amino acid protein that features a N-terminal Pyrin-like domain (PYD) and a C-terminal caspase recruitment domain (CARD), which are essential for its role in apoptotic and inflammatory signaling pathways. The structural configuration of ASC allows ASC to facilitate interactions between adaptor proteins such as Apaf-1 and pro-caspases like CASP9, thereby promoting the activation of caspases that execute programmed cell death. Additionally, the PYD and CARD domains enable ASC to assemble into larger protein complexes, such as inflammasomes, which are critical for the regulation of immune responses and the maintenance of cellular homeostasis. Understanding the intricate structure of ASC is fundamental to elucidating ASC function in apoptosis and inflammation, making anti-ASC antibody (D-3) an invaluable tool for researchers investigating these complex biological processes. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, ASC (D-3) antibody provides versatile applications for detailed structural and functional studies of ASC in diverse research settings.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ASC/TMS1/PYCARD Antibody (D-3) References:

1. ASC, which is composed of a PYD and a CARD, is up-regulated by inflammation and apoptosis in human neutrophils.  |  Shiohara, M., et al. 2002. Biochem Biophys Res Commun. 293: 1314-8. PMID: 12054656
2. ASC is an activating adaptor for NF-kappa B and caspase-8-dependent apoptosis.  |  Masumoto, J., et al. 2003. Biochem Biophys Res Commun. 303: 69-73. PMID: 12646168
3. Asc modulates the function of NLRC4 in response to infection of macrophages by Legionella pneumophila.  |  Case, CL. and Roy, CR. 2011. mBio. 2: PMID: 21771913
4. Novel role of ASC as a regulator of metastatic phenotype.  |  Okada, N., et al. 2016. Cancer Med. 5: 2487-500. PMID: 27350283
5. Silencing of ASC in Cutaneous Squamous Cell Carcinoma.  |  Meier, K., et al. 2016. PLoS One. 11: e0164742. PMID: 27768771
6. Detection of ASC Oligomerization by Western Blotting.  |  Lugrin, J. and Martinon, F. 2017. Bio Protoc. 7: PMID: 29085861
7. The central inflammasome adaptor protein ASC activates the inflammasome after transition from a soluble to an insoluble state.  |  Prather, ER., et al. 2022. J Biol Chem. 298: 102024. PMID: 35568196
8. Directly targeting ASC by lonidamine alleviates inflammasome-driven diseases.  |  Chen, C., et al. 2022. J Neuroinflammation. 19: 315. PMID: 36577999
9. ASC specks exacerbate α‑synuclein pathology via amplifying NLRP3 inflammasome activities.  |  Zheng, R., et al. 2023. J Neuroinflammation. 20: 26. PMID: 36740674
10. Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence.  |  Jenster, L., et al. 2023. Methods Mol Biol. 2696: 73-92. PMID: 37578716

The preferred ASC/TMS1/PYCARD antibody is ASC/TMS1/PYCARD Antibody (F-9): sc-271054.