Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ASC-1 CRISPR/Cas9 KO Plasmid (m): sc-425141
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Asc-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Asc-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ASC-1 Antibody (F-8): sc-365202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASC-1 CRISPR/Cas9 KO Plasmid (m) | sc-425141 | 20 µg | $397.00 |
Overview
Trip4 encodes ASC-1, a transcriptional coactivator that functions within the Activating Signal Cointegrator-1 (ASC-1) complex to couple sequence-specific transcription factors with RNA polymerase II machinery. ASC-1 contributes to regulation of gene expression programs linked to nuclear receptor signaling and broader transcriptional control, including processes that influence differentiation, metabolism, and cellular stress responses. In mouse systems, Trip4-dependent transcriptional modulation is used to dissect how coactivators integrate signaling inputs to shape chromatin-associated transcriptional outputs. Altered ASC-1 complex function has been associated with dysregulated developmental and neuromuscular gene programs, supporting its relevance for studying disease-associated transcriptional networks without implying clinical outcomes.
Asc-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Trip4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Trip4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Trip4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Asc-1 protein expression.
This CRISPR knockout system enables efficient generation of Trip4-deficient cell models for investigation of Asc-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Trip4 exon(s) critical for Asc-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Trip4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Asc-1 CRISPR/Cas9 KO Plasmid (m) and Asc-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Trip4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Asc-1 HDR Plasmid (m) and Asc-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Trip4 homology arms to support homology-directed repair at defined Trip4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.