Anticorps Annexin VI (E-5): sc-271859
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- Anti-L'anticorps Annexin VI E-5 est un monoclonal IgG1 κ L'Anticorps Annexin VI, cité dans 4 publications, fourni en 200 µg/ml
- spécifique d'un épitope situé entre les acides aminés 5-27 au niveau N-terminus de Annexin VI d'origine human
- Anti-L'Anticorps Annexin VI (E-5) est recommandé pour la détection de Annexin VI d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
- Anti-L'Anticorps Annexin VI (E-5) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Annexin VI (E-5): sc-271859.
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Annexin VI Antibody (E-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with Annexin VI Antibody (E-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
ACCÈS RAPIDE AUX LIENS
VOIR ÉGALEMENT...
- Anticorps supplémentaires Annexin dont Annexin A10, Annexin A13, Annexin A9, Annexin I, Annexin II, Annexin III, Annexin IV, Annexin V, Annexin VI et Annexin VII
- Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional
I'm interested in testing this (sc-271859 ) antibody on zebrafish and would like to ask if it is possible to get a test sample?
Thank you for your question. Please contact our technical service team to arrange for a free sample of this antibody.
Date de publication: 2022-03-25
What application is the blocking peptide sc-271859 P appropriate for?
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Date de publication: 2017-02-27
Rated 5 de
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mcm77 par
Ottimo funzionamento!!!
L’anticorpo anti-Annexin VI (sc-271859), richiesto con l’Ordine n° 535 dell’11/11/2021 e arrivato il giorno 23/11/2021, è stato testato su un lisato di cellule HeLa, ottenuto tramite omogenizzazione manuale in una soluzione di PBS-0.1% Igepal contenente un cocktail di inibitori di proteasi. Aliquote di lisato da 2 μg, 5 μg, 10 μg e 20 μg di proteine sono state caricate su un gel di poliacrilammide al 10% e sottoposte a Western Blotting. La membrana così ottenuta è stata sottoposta a blocking (in una soluzione al 5% di non-fat dried milk in TBS-0.05% TWEEN 20) e, successivamente, incubata con l’anticorpo primario sopra riportato (1:1000 vol/vol). La membrana è poi stata incubata con un anticorpo secondario anti-mouse (1:1500 vol/vol) coniugato con perossidasi di rafano.
I segnali di chemiluminescenza sono stati rivelati tramite il sistema di imaging digitale Amersham Imager 680 (GE Healthcare Life Sciences).
Come mostrato nell’immagine di seguito riportata, l’anticorpo produce segnali in corrispondenza del peso molecolare dell’Annessina VI in maniera concentrazione dipendente.
Date de publication: 2021-11-25
Rated 5 de
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Jianwen par
This antibody is good for WB and IP
I got this antibody few month before, it is good for both WB and IP
Date de publication: 2017-11-20
Rated 5 de
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wenbin77 par
works great on western blot and IHC
We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date de publication: 2017-09-20
Rated 4 de
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jm23 par
WORKED WELL
I received this antibody as a free trial sample and used for WB and Immunoprecipitation. It worked well.
Date de publication: 2017-09-11
Rated 5 de
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FL par
Distinct band observed via WB using product sc
Distinct band observed via WB using product sc-271859 using HeLa whole cell lysate. -SCBT QC
Date de publication: 2015-04-12
Rated 5 de
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ET par
Good specificity shown by using transfected
Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VIII protein via Western Blot analysis. -SCBT QC
Date de publication: 2015-03-29
Rated 5 de
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QZ par
Positive detection of endogenous Annexin
Positive detection of endogenous Annexin VIII in JAR cells by WB analysis. -SCBT QC
Date de publication: 2015-03-21
Rated 5 de
5
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JT par
Produced excellent immunofluorescence membrane
Produced excellent immunofluorescence membrane and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date de publication: 2015-02-26