Anticorps alpha 1A-AR (4D8) recommandé pour la détection de α1A-AR d'origine mouse, rat et human par WB, IP et ELISA

Anticorps α1A-AR (4D8): sc-100291

Anticorps α1A-AR (4D8) est évalué 4.7 de 5 de 3.
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Fiches techniques

    • Anti-L'anticorps alpha 1A-AR 4D8 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps α1A-AR fourni en 100 µg/ml
    • raised against an N-terminal region of α1A-AR of human origin
    • recommandé pour la détection de α1A-AR d'origine mouse, rat et human par WB, IP et ELISA
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de α1A-AR (4D8): sc-100291.

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) "How reliable are G-protein-coupled receptor antibodies?" NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

Posée par: cpoon
Thank you for your question. The manuscript you mention recommends validating by IHC, but I would like to aslso suggest that also using siRNAs and CRISPR followed by a WB would also provide validation results to determine the reliability of the antibody. You can find recommended gene silencing products on the antibody product block: https://www.scbt.com/scbt/product/alpha1a-ar-antibody-4d8?requestFrom=search If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Répondue par: Tech Service
Date de publication: 2017-06-28

For Western Blot, is it recommended to use denatured or non-denatured conditions with α1A-AR (4D8): sc-100291 antibody?

Posée par: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Répondue par: Technical Support
Date de publication: 2017-03-27
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Rated 5 de 5 de par Worked very well I used this antibody on mice tissues (liver, muscle, aorta) and it worked very well.
Date de publication: 2018-04-06
Rated 5 de 5 de par positive results Product sc-100291 is used for kinase assay using human myeloid cells,positive results!
Date de publication: 2016-12-17
Rated 4 de 5 de par Distinct band observed by WB using HL Distinct band observed by WB using HL-60 whole cell lysate. -SCBT QC
Date de publication: 2014-02-27
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