α-Actin Antibody (SPM332): sc-56499

α-Actin Antibody (SPM332) is a mouse monoclonal IgG2a kappa light chain antibody that detects smooth muscle α-Actin from mouse, rat, human, and bovine sources through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). α-Actin Antibody (SPM332) comes in a non-conjugated form for flexible research applications. Actin maintains cell shape, enables motility, and facilitates intracellular transport. α-Actin exists in a dynamic balance between monomeric globular actin (G-actin) and filamentous actin (F-actin), allowing quick assembly and disassembly in response to cellular signals. This adaptability is crucial for muscle contraction and cell division, where precise actin regulation ensures proper function. α-Actin is predominantly expressed in muscle cells, unlike β-Actin and γ-Actin found in non-muscle tissues. Small GTPases and other signaling pathways regulate α-Actin organization in the cytoskeleton, highlighting α-Actin′s significance in muscle function and cellular processes.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-Actin Antibody (SPM332) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-α-Actin Antibody (SPM332) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-Actin Antibody (SPM332) Specifications

Alternate Product Name: ACTA2

Product Clone Name: SPM332

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-10

Epitope Target: α-Actin

Epitope Species: human

Product Reactivity/Detection: smooth muscle α-Actin

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for α-Actin Antibody (SPM332): N/A

The preferred α-Actin antibody is α-Actin Antibody (1A4): sc-32251.