Anticorps alpha-Actin (alpha-SM1) recommandé pour la détection de smooth muscle α-Actin d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps α-Actin (alpha-SM1): sc-130617

Anticorps α-Actin (alpha-SM1) est évalué 4.4 de 5 de 5.
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Fiches techniques

    • L'Anticorps α-Actin (alpha-SM1) est un monoclonal de souris IgG2a (chaîne légère kappa) fourni en 200 µg/ml
    • raised against an N-terminal decapeptide of smooth muscle α-Actin of human origin
    • recommandé pour la détection de smooth muscle α-Actin d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; non cross-reactif avec actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
    • disponible conjugué à l'agarose pour IP
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Voir α-Actin (1A4): sc-32251 pour d'autres anticorps conjugués α-Actin, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.

Can this α-Actin Antibody (alpha-SM1):sc-130617 be used to stain α-smooth muscle actin in vascular endothelial cell?

Posée par: ny1164
Thank you for your question. We have tested this antibody in vascular endothelial cells so we can not confirm reactivity in this cell type.
Répondue par: Technical Support
Date de publication: 2019-01-25

Can α-Actin (alpha-SM1): sc-130617 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: TinTin
Thank you for your question. Yes, α-Actin (alpha-SM1): sc-130617 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-02-15
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Rated 4 de 5 de par Activation of Adiponectin Receptor Regulates Propr We used the anti-SMA antibody to determine expression of smooth muscle actin in the atortic root cross section by immunofluorescent staining, and obtained a good results. We presented the results as Figure 6D in our published paper.
Date de publication: 2017-08-17
Rated 5 de 5 de par Great reactivity A great performing antibody in blotting and staining procedures. It gives off a clear and specific signal and has great reactivity. This antibody has been used to publish more than 25 times.
Date de publication: 2017-02-07
Rated 5 de 5 de par It worked well in WB It worked well in WB, IHC, IF.
Date de publication: 2015-10-13
Rated 4 de 5 de par Western blot data with human aortic SMCs Western blot data with human aortic SMCs. -SCBT QC
Date de publication: 2013-05-01
Rated 4 de 5 de par Decent bands in Western with rat pulmonary Decent bands in Western with rat pulmonary fibroblasts. -SCBT QC
Date de publication: 2010-03-26
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