Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h): sc-401251-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h) and Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADCY6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h) | sc-401251-NIC | 20 µg | $410.00 | |||
Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h2) | sc-401251-NIC-2 | 20 µg | $410.00 |
Human ADCY6 encodes adenylate cyclase 6 (AC6), a membrane-associated enzyme that converts ATP to cAMP downstream of G protein–coupled receptors, integrating signals from Gαs- and Gαi-coupled pathways. AC6-derived cAMP regulates PKA and EPAC signaling, shaping phosphorylation networks that influence ion transport, contractility, metabolic control, and stimulus-dependent transcriptional programs such as CREB. As a key effector of receptor-driven second-messenger production, ADCY6 is commonly examined in contexts involving dysregulated GPCR signaling and altered cAMP homeostasis, including cardiovascular and airway biology as well as endocrine and metabolic phenotypes. Its compartmentalized activity at the plasma membrane makes it useful for studying signaling microdomains and cross-talk with calcium and phosphodiesterase pathways.
Adenylate cyclase 6/AC6/ADCY6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADCY6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADCY6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADCY6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADCY6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.