ADAM28 Antibody (A-5): sc-393877

ADAM28 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADAM28 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ADAM28 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADAM28, a member of the ADAM (a disintegrin and metalloprotease) family, plays a crucial role in mediating cell adhesion, particularly through interaction with integrin α4β1, which is essential for various physiological processes such as immune response and tissue development. ADAM28 is predominantly expressed on the cell surface of lymphocytes and in the epididymis, highlighting importance in reproductive biology and immune function. ADAM28 includes several functional domains, such as a metalloprotease domain and a disintegrin domain, which facilitate enzymatic activity and interactions with other proteins. The gene encoding human ADAM28 is located on chromosome 8p12, and unique evolutionary relationship to snake venom metalloproteases suggests potential insights into functional mechanisms and therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ADAM28 Antibody (A-5) References:

1. Structure-function analysis of the ADAM family of disintegrin-like and metalloproteinase-containing proteins (review).  |  Stone, AL., et al. 1999. J Protein Chem. 18: 447-65. PMID: 10449042
2. The ADAM gene family: surface proteins with adhesion and protease activity.  |  Primakoff, P. and Myles, DG. 2000. Trends Genet. 16: 83-7. PMID: 10652535
3. Cloning and characterization of ADAM28: evidence for autocatalytic pro-domain removal and for cell surface localization of mature ADAM28.  |  Howard, L., et al. 2000. Biochem J. 348 Pt 1: 21-7. PMID: 10794709
4. Catalytic activity of ADAM28.  |  Howard, L., et al. 2001. FEBS Lett. 498: 82-6. PMID: 11389903
5. The lymphocyte metalloprotease MDC-L (ADAM 28) is a ligand for the integrin alpha4beta1.  |  Bridges, LC., et al. 2002. J Biol Chem. 277: 3784-92. PMID: 11724793
6. The ADAMDEC1 (decysin) gene structure: evolution by duplication in a metalloprotease gene cluster on chromosome 8p12.  |  Bates, EE., et al. 2002. Immunogenetics. 54: 96-105. PMID: 12037602
7. [Research advances on ADAM28 expression and ADAM28-mediated tumor metastasis].  |  Zhu, XL., et al. 2014. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 22: 1142-7. PMID: 25130845
8. ADAM28 dramatically regulates the biological features of human gingival fibroblasts.  |  Zhao, Z., et al. 2019. Odontology. 107: 333-341. PMID: 30552542
9. Oncogenic ADAM28 induces gemcitabine resistance and predicts a poor prognosis in pancreatic cancer.  |  Wei, L., et al. 2019. World J Gastroenterol. 25: 5590-5603. PMID: 31602160
10. ADAM28: Another ambivalent protease in cancer.  |  Hubeau, C., et al. 2020. Cancer Lett. 494: 18-26. PMID: 32861707
11. CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response.  |  Wu, Z., et al. 2022. Front Immunol. 13: 865596. PMID: 35634306
12. ADAM, a novel family of membrane proteins containing A Disintegrin And Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions.  |  Wolfsberg, TG., et al. 1995. J Cell Biol. 131: 275-8. PMID: 7593158

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ADAM28 Antibody (A-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ADAM28 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ADAM28 Antibody (A-5) Specifications

Alternate Product Name: ADAM28

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 366-420

Epitope Target: ADAM28

Epitope Species: human

Product Reactivity/Detection: ADAM28

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ADAM28 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ADAM28 antibody is ADAM28 Antibody (H-4): sc-514228.