AAK1 Antibody (LW-M11): sc-134242

AAK1 Antibody (LW-M11) is a mouse monoclonal IgG2a kappa light chain antibody that detects the AAK1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-AAK1 antibody (LW-M11) is available as the non-conjugated format. AAK1, or adaptor-associated kinase 1, is a crucial protein that plays a significant role in receptor-mediated endocytosis, a process essential for cellular communication and nutrient uptake. This 961 amino acid protein, which belongs to the protein kinase superfamily and the serine/threonine protein kinase family, is encoded by a gene located on human chromosome 2p14. AAK1 is involved in ATP and nucleotide binding, protein serine/threonine kinase functions, and transferase activity, and specifically phosphorylates a subunit of the adaptor protein complex 2 (AP-2). This phosphorylation enhances the binding of AP-2 to sorting signals in membrane-bound receptors, thereby facilitating endocytosis. AAK1 contains a single protein kinase domain, and clathrin stimulates kinase activity, allowing AAK1 to exist in two alternatively spliced isoforms, which may have distinct regulatory roles in cellular processes.

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AAK1 Antibody (LW-M11) References:

1. Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals.  |  Ricotta, D., et al. 2002. J Cell Biol. 156: 791-5. PMID: 11877457
2. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.  |  Conner, SD. and Schmid, SL. 2002. J Cell Biol. 156: 921-9. PMID: 11877461
3. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton.  |  Smythe, E. and Ayscough, KR. 2003. EMBO Rep. 4: 246-51. PMID: 12634840
4. Kinases in clathrin-mediated endocytosis.  |  Korolchuk, V. and Banting, G. 2003. Biochem Soc Trans. 31: 857-60. PMID: 12887321
5. Differential requirements for AP-2 in clathrin-mediated endocytosis.  |  Conner, SD. and Schmid, SL. 2003. J Cell Biol. 162: 773-9. PMID: 12952931
6. The serine/threonine kinase cyclin G-associated kinase regulates epidermal growth factor receptor signaling.  |  Zhang, L., et al. 2004. Proc Natl Acad Sci U S A. 101: 10296-301. PMID: 15240878
7. Unlocking the mysteries of Na+-K+-ATPase endocytosis: phosphorylation is the key.  |  Collawn, JF. 2006. Am J Respir Cell Mol Biol. 35: 1-2. PMID: 16782842
8. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway.  |  Henderson, DM. and Conner, SD. 2007. Mol Biol Cell. 18: 2698-706. PMID: 17494869
9. Kinase requirements in human cells: II. Genetic interaction screens identify kinase requirements following HPV16 E7 expression in cancer cells.  |  Baldwin, A., et al. 2008. Proc Natl Acad Sci U S A. 105: 16478-83. PMID: 18948598

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AAK1 Antibody (LW-M11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-AAK1 Antibody (LW-M11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AAK1 Antibody (LW-M11) Specifications

Alternate Product Name: AAK1

Product Clone Name: LW-M11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 315-415

Epitope Target: AAK1

Epitope Species: human

Product Reactivity/Detection: AAK1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for AAK1 Antibody (LW-M11): N/A