Date published: 2026-6-5

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5-Methylcytidine (CAS 2140-61-6)

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Application:
5-Methylcytidine is a derivative of Cytidine
CAS Number:
2140-61-6
Purity:
≥98%
Molecular Weight:
257.24
Molecular Formula:
C10H15N3O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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5-Methylcytidine (5-mC) stands as a modified nucleoside widely present in various organisms, including humans. Originating from cytidine, a nucleoside found in both DNA and RNA, 5-Methylcytidine holds significance as a epigenetic modification. This modification possesses the ability to impact gene expression without inducing alterations to the underlying genetic sequence. Through its involvement in DNA methylation, 5-Methylcytidine plays a pivotal role in processes that can influence gene expression, ultimately influencing the phenotype of an organism. Within the realm of epigenetic modifications, 5-Methylcytidine plays a role in various processes, notably including DNA methylation. DNA methylation involves the addition of a methyl group to a cytosine nucleotide in DNA, thereby influencing gene expression. This biochemical process is facilitated by DNA methyltransferases (DNMTs), which recognize and transfer a methyl group to 5-Methylcytidine, leading to the formation of 5-methylcytosine. The resulting methylation can impact gene expression by impeding gene transcription or modifying the structure of chromatin.


5-Methylcytidine (CAS 2140-61-6) References

  1. tRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae.  |  Preston, MA., et al. 2013. RNA. 19: 243-56. PMID: 23249748
  2. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs.  |  Edelheit, S., et al. 2013. PLoS Genet. 9: e1003602. PMID: 23825970
  3. Efficient deamination of 5-methylcytidine and 5-substituted cytidine residues in DNA by human APOBEC3A cytidine deaminase.  |  Suspène, R., et al. 2013. PLoS One. 8: e63461. PMID: 23840298
  4. Formation and abundance of 5-hydroxymethylcytosine in RNA.  |  Huber, SM., et al. 2015. Chembiochem. 16: 752-5. PMID: 25676849
  5. N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice.  |  Andries, O., et al. 2015. J Control Release. 217: 337-44. PMID: 26342664
  6. A novel malic acid-enhanced method for the analysis of 5-methyl-2'-deoxycytidine, 5-hydroxymethyl-2'-deoxycytidine, 5-methylcytidine and 5-hydroxymethylcytidine in human urine using hydrophilic interaction liquid chromatography-tandem mass spectrometry.  |  Guo, C., et al. 2018. Anal Chim Acta. 1034: 110-118. PMID: 30193624
  7. Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate.  |  Yuan, F., et al. 2019. Chem Commun (Camb). 55: 2328-2331. PMID: 30723849
  8. The emerging role of RNA modifications in the regulation of mRNA stability.  |  Boo, SH. and Kim, YK. 2020. Exp Mol Med. 52: 400-408. PMID: 32210357
  9. Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.  |  Selmi, T., et al. 2021. Nucleic Acids Res. 49: 1006-1022. PMID: 33330931
  10. The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer.  |  Wang, JZ., et al. 2021. Cancer Commun (Lond). 41: 560-575. PMID: 33991457
  11. Regulation of antiviral innate immunity by chemical modification of viral RNA.  |  Li, N. and Rana, TM. 2022. Wiley Interdiscip Rev RNA. 13: e1720. PMID: 35150188
  12. Epitranscriptomic dynamics in brain development and disease.  |  Shafik, AM., et al. 2022. Mol Psychiatry. 27: 3633-3646. PMID: 35474104
  13. Comprehensive Analysis of m5C Methylation Regulatory Genes and Tumor Microenvironment in Prostate Cancer.  |  Yu, G., et al. 2022. Front Immunol. 13: 914577. PMID: 35757739
  14. Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases.  |  Arguello, AE., et al. 2022. Nat Commun. 13: 4176. PMID: 35853884
  15. RNA modifications in aging-associated cardiovascular diseases.  |  Yang, X., et al. 2022. Aging (Albany NY). 14: 8110-8136. PMID: 36178367

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

5-Methylcytidine, 25 mg

sc-221036
25 mg
$81.00

5-Methylcytidine, 100 mg

sc-221036A
100 mg
$321.00