5-(Hydroxymethyl)-2′-deoxycytidine-d3 (CAS 7226-77-9 (unlabeled))

5-(Hydroxymethyl)-2′-deoxycytidine-d3 is a deuterated analog of 5-(Hydroxymethyl)-2′-deoxycytidine, a compound that is used in the area of epigenetics and nucleic acids research. The incorporation of deuterium atoms increases the molecular stability and can be utilized to study the compound′s metabolism and dynamics within biological systems using mass spectrometry or NMR spectroscopy. This deuterated variant is particularly valuable in the examination of DNA modifications and repair mechanisms, as the hydroxymethyl group on the cytidine base is a known intermediate in the oxidation of 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine, both of which are involved in active DNA demethylation processes. By tracing the incorporation and fate of 5-(Hydroxymethyl)-2′-deoxycytidine-d3 in genomic DNA, researchers can gain insights into the role of hydroxymethylcytosine in gene expression regulation and the implications of DNA hydroxymethylation in development and disease.