CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.
CRISPR/dCas9 Activation Plasmids consist of three plasmids at a 1:1:1 mass ratio. The second plasmid encodes the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene.
Produits CRISPR de Knockout et d'Activation Cre Vector (): sc-418923
20 µg d'ADN plasmatique purifié et prêt-à-transfecter; Permet jusqu'à 20 transfections
Le Vecteur Cre exprime la recombinase Cre catalysant la recombinaison des sites spécifiques d'ADN entre les deux sites LoxP
Le vecteur Cre est conçu pour cibler spécifiquement les sites LoxP flanquant les marqueurs de sélection insérés par le plasmide HDR et pour exciser le matériel génétique.
Le Vecteur Cre contient un promoteur CMV pour diriger l'expression de la recombinase Cre
Après transfection, les cellules peuvent être testées par WB, IF ou IHC en utilisant un anticorps contre le gène cible, ou peuvent être re-transfectées pour une autre modification de l'ADN
For assistance please contact Technical service . Please call 800-457-3801 or email scbt@scbt.com.
Répondue par: BlakeJ
Date de publication: 2026-01-21
Hi, can the above plasmid be used for yeast namely saccharomyces cerevisiae?
Posée par: NT777
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Répondue par: lai13
Date de publication: 2024-07-25
How to transfect the cre-vector to the cells that already have puromycin gene incorporated by HDR vector? transfect, then wait two or three days and consider those cells puromycin free? correct? do I select?
Posée par: michaela
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Répondue par: lai13
Date de publication: 2024-07-25
Can you provide the plasmid map and size of the vector?
Thanks,
Posée par: Chris1
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Répondue par: lai13
Date de publication: 2024-07-25
Does this kit includes a vector to flanked my target gene?
Posée par: Ldas01
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Répondue par: Blake J
Date de publication: 2022-01-26
How large is this plasmid?
Posée par: Dkr0
Thank you for your question. Our Cre Vector sc-418923 has a size of approximately 5.9 kilobases.
Répondue par: Tech Support Europe
Date de publication: 2024-04-20
Why is CRE Vector Transfection "Recommended" after for CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection (following selection)? Is it simply to remove the selection marker? Or, does it help in any way with knocking out the gene?
Posée par: jmd6207
Thank you for your question. It is not mandatory, and only recommended if you wish to removed the puromycin selection marker. Removing the HDR plasmid insert using the CRE vector does not help with knocking out the gene. It is useful if you wish you knock out a second gene or if you need you cells to no longer be puromycin resistant.
Répondue par: Tech Service
Date de publication: 2024-03-19
whats the cre loxp systems
Posée par: waleed xel
Thank you for your question. The Cre-Lox system is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. You can find our more about the Cre-Lox system at any of the links below:
https://en.wikipedia.org/wiki/Cre-Lox_recombination
https://www.youtube.com/watch?v=oLPjiwM0G7A&app=desktop
Rated 5 de
5 de
Jelena par
Very useful product.Very useful tool to achieve double or triple knock-out with the CRISPR system since the most of Santa Cruz CRISPR products are resistant to the same antibiotic-pyromycin.
Date de publication: 2017-04-25
Rated 4 de
5 de
mask par
Remove the marker gene efficientlyI used this vector to remove the neo marker-gene in the pig primary cells . The marker-free efficiency was so high to 80% .I nucleofected the 4ug Cre vector to the 1000000 cells by ALONZO. But, i suggested that can the CAG promoter may replace the CMV promoter of this vector, because of the CMV promoter may Cause gene-silencing in some special cell lines