XPC Antibody (D-10): sc-74410

XPC Antibody (D-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects XPC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-XPC antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XPC protein plays a crucial role in the nucleotide excision repair (NER) pathway, which maintains genomic stability by recognizing and repairing bulky DNA lesions, such as those caused by ultraviolet light. Deficiencies in this repair mechanism can lead to severe consequences, including an increased risk of skin cancers, particularly in individuals with Xeroderma Pigmentosum (XP), a genetic disorder characterized by extreme sensitivity to sunlight. XPC gene, located on chromosome 3p25, encodes a protein that forms a complex with Cen2 and HR23B, which are vital for stabilizing XPC and facilitating DNA damage recognition. The carboxy-terminus of XPC is critical for binding to HR23B and damaged DNA; mutations that truncate this region can result in nonfunctional XPC proteins, further underscoring XPC′s importance in the DNA repair process.

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XPC Antibody (D-10) References:

1. Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair.  |  Araki, M., et al. 2001. J Biol Chem. 276: 18665-72. PMID: 11279143
2. Complementation of the DNA repair deficiency in human xeroderma pigmentosum group a and C cells by recombinant adenovirus-mediated gene transfer.  |  Muotri, AR., et al. 2002. Hum Gene Ther. 13: 1833-44. PMID: 12396616
3. The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH.  |  Uchida, A., et al. 2002. DNA Repair (Amst). 1: 449-61. PMID: 12509233
4. A molecular mechanism for DNA damage recognition by the xeroderma pigmentosum group C protein complex.  |  Sugasawa, K., et al. 2002. DNA Repair (Amst). 1: 95-107. PMID: 12509299
5. Global genome repair is required to activate KIN17, a UVC-responsive gene involved in DNA replication.  |  Masson, C., et al. 2003. Proc Natl Acad Sci U S A. 100: 616-21. PMID: 12525703
6. XPC beyond nucleotide excision repair and skin cancers.  |  Zebian, A., et al. 2019. Mutat Res Rev Mutat Res. 782: 108286. PMID: 31843141
7. XPC multifaceted roles beyond DNA damage repair: p53-dependent and p53-independent functions of XPC in cell fate decisions.  |  Zebian, A., et al. 2022. Mutat Res Rev Mutat Res. 789: 108400. PMID: 35690409
8. XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair.  |  Blessing, C., et al. 2022. Nat Commun. 13: 4762. PMID: 35963869
9. Lesion recognition by XPC, TFIIH and XPA in DNA excision repair.  |  Kim, J., et al. 2023. Nature. 617: 170-175. PMID: 37076618
10. Assignment of xeroderma pigmentosum group C (XPC) gene to chromosome 3p25.  |  Legerski, RJ., et al. 1994. Genomics. 21: 266-9. PMID: 8088800
11. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
12. Overproduction, purification, and characterization of the XPC subunit of the human DNA repair excision nuclease.  |  Reardon, JT., et al. 1996. J Biol Chem. 271: 19451-6. PMID: 8702634

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XPC Antibody (D-10) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-XPC Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XPC Antibody (D-10) Specifications

Alternate Product Name: XPC

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 641-940

Epitope Target: XPC

Epitope Species: human

Product Reactivity/Detection: XPC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for XPC Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin