VMP1 CRISPR/Cas9 KO Plasmid (h): sc-405940
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VMP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VMP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VMP1 CRISPR/Cas9 KO Plasmid (h) | sc-405940 | 20 µg | $397.00 |
Overview
VMP1 (vacuole membrane protein 1) is an integral membrane protein required for multiple membrane remodeling events, most notably the initiation and progression of autophagy. It participates in phagophore formation through interactions with autophagy machinery, supports autophagosome biogenesis, and has been implicated in ER–organelle contact site dynamics that coordinate lipid transfer and organelle homeostasis. Beyond autophagy, VMP1 contributes to vesicular trafficking and cellular stress adaptation, processes frequently perturbed in cancer, neurodegeneration, and inflammatory contexts where altered proteostasis and organelle turnover are prominent features.
VMP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VMP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VMP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VMP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VMP1 protein expression.
This CRISPR knockout system enables efficient generation of VMP1-deficient cell models for investigation of VMP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VMP1 exon(s) critical for VMP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VMP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VMP1 CRISPR/Cas9 KO Plasmid (h) and VMP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VMP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VMP1 HDR Plasmid (h) and VMP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VMP1 homology arms to support homology-directed repair at defined VMP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.