VILIP-1 Antibody (183B2E): sc-517633

VILIP-1 Antibody (183B2E) is a mouse monoclonal IgG1 antibody that detects VILIP-1 in human samples through western blotting (WB). VILIP-1, or visinin-like protein 1, is encoded by the VSNL1 gene located on the short arm of chromosome 2 and is a member of the neuronal calcium sensor family, which is part of the larger calcium-binding protein superfamily. VILIP-1 is characterized by four EF-hands and a double-stranded RNA-binding domain, which are crucial for calcium signaling within neurons. VILIP-1 is predominantly expressed in the membrane, cytoplasm, and cytoskeleton of sympathetic and parasympathetic neurons throughout the brain, with notable exceptions in the caudate-putamen region. Expression levels significantly decline with age, which may have implications for neuronal health and function. VILIP-1 interacts with actin in the cytoskeleton, facilitating translocation to the membrane, where VILIP-1 plays a vital role in modulating calcium-dependent signaling pathways. Additionally, VILIP-1 binds to the 3′-untranslated region of trkB double-stranded mRNA in a calcium-dependent manner, influencing neurotrophic signaling. VILIP-1 also associates with G protein-receptor kinase 1, inhibiting membrane binding, which suggests a regulatory role in G-protein signaling cascades. Notably, decreased levels of VILIP-1 have been observed in the brains of individuals with Alzheimer′s disease, indicating potential involvement in the pathophysiology of neurodegenerative disorders.

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VILIP-1 Antibody (183B2E) References:

1. Calcium-myristoyl switch, subcellular localization, and calcium-dependent translocation of the neuronal calcium sensor protein VILIP-3, and comparison with VILIP-1 in hippocampal neurons.  |  Spilker, C. and Braunewell, KH. 2003. Mol Cell Neurosci. 24: 766-78. PMID: 14664824
2. VILIP-1 downregulation in non-small cell lung carcinomas: mechanisms and prediction of survival.  |  Fu, J., et al. 2008. PLoS One. 3: e1698. PMID: 18301774
3. Neuronal Ca2+ sensor VILIP-1 leads to the upregulation of functional alpha4beta2 nicotinic acetylcholine receptors in hippocampal neurons.  |  Zhao, CJ., et al. 2009. Mol Cell Neurosci. 40: 280-92. PMID: 19063970
4. Structural analysis of Mg2+ and Ca2+ binding, myristoylation, and dimerization of the neuronal calcium sensor and visinin-like protein 1 (VILIP-1).  |  Li, C., et al. 2011. J Biol Chem. 286: 6354-66. PMID: 21169352
5. Involvement of VILIP-1 (visinin-like protein) and opposite roles of cyclic AMP and GMP signaling in in vitro cell migration of murine skin squamous cell carcinoma.  |  Schönrath, K., et al. 2011. Mol Carcinog. 50: 319-33. PMID: 21480386
6. CSF VILIP-1 predicts rates of cognitive decline in early Alzheimer disease.  |  Tarawneh, R., et al. 2012. Neurology. 78: 709-19. PMID: 22357717
7. The visinin-like proteins VILIP-1 and VILIP-3 in Alzheimer's disease-old wine in new bottles.  |  Braunewell, KH. 2012. Front Mol Neurosci. 5: 20. PMID: 22375104
8. APOE ε4 Allele Is Associated with Elevated Levels of CSF VILIP-1 in Preclinical Alzheimer's Disease.  |  Wang, L., et al. 2020. Neuropsychiatr Dis Treat. 16: 923-931. PMID: 32308396
9. Concordance between the In Vivo Content of Neurospecific Proteins (BDNF, NSE, VILIP-1, S100B) in the Hippocampus and Blood in Patients with Epilepsy.  |  Tikhonova, MA., et al. 2023. Int J Mol Sci. 25: PMID: 38203674
10. Dynamics of synaptic damage in severe traumatic brain injury revealed by cerebrospinal fluid SNAP-25 and VILIP-1.  |  Olde Heuvel, F., et al. 2024. J Neurol Neurosurg Psychiatry. 95: 1158-1167. PMID: 38825349

The preferred VILIP-1 antibody is VILIP-1 Antibody (2F1-E3): sc-293209.