VDAC3 CRISPR/Cas9 KO Plasmid (m): sc-423663
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VDAC3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VDAC3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VDAC3 CRISPR/Cas9 KO Plasmid (m) | sc-423663 | 20 µg | $397.00 |
Overview
Vdac3 encodes voltage-dependent anion channel 3 (VDAC3), a β-barrel pore in the mitochondrial outer membrane that regulates metabolite and ion exchange between the cytosol and mitochondria. As part of the VDAC family, VDAC3 contributes to oxidative phosphorylation coupling, mitochondrial membrane potential maintenance, and redox-sensitive control of mitochondrial homeostasis. VDAC3-associated processes intersect with apoptosis signaling, reactive oxygen species handling, and mitochondrial quality control pathways that shape cellular stress responses. Altered VDAC3 function has been studied in contexts involving mitochondrial dysfunction, including bioenergetic imbalance and tissue-specific defects linked to metabolic and neurodegenerative disease mechanisms.
VDAC3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vdac3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vdac3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vdac3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VDAC3 protein expression.
This CRISPR knockout system enables efficient generation of Vdac3-deficient cell models for investigation of VDAC3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vdac3 exon(s) critical for VDAC3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vdac3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VDAC3 CRISPR/Cas9 KO Plasmid (m) and VDAC3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vdac3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VDAC3 HDR Plasmid (m) and VDAC3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vdac3 homology arms to support homology-directed repair at defined Vdac3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.