UDP-GlcDH Anticuerpo (A-9) recomendado para detectarUDP-GlcDH de mouse, rat y human origen, mediante WB, IP, IF y ELISA

UDP-GlcDH Anticuerpo (A-9): sc-393438

UDP-GlcDH Anticuerpo (A-9) clasificado 5.0 de 5 por 2.
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Ficha técnica

    • UDP-GlcDH Anticuerpo (A-9) es un monoclonal de ratón IgG1 (kappa light chain) proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 459-486 en el C-terminus de UDP-GlcDH de origen human
    • recomendado para detectarUDP-GlcDH de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • péptido de bloqueo, sc-393438 P
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.

What blotting conditions were used for the blot? dilution? blocking agent? I used the antibody at 1:1000 in 5% milk/TBST. It was almost clean enough but could be a little better.

Preguntado por: Joy N
Thank you for your question. You can find dilution recommendations for each antibody on their respective datasheets. Our protocol for western blotting that is used by our labs as well can be found under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting We recommend UltraCruz® Blocking Reagent: sc-516214. Alternatively, a milk-based blocking buffer will produce a cleaner blot while BSA will provide stronger signals. Incubating the blot at a low dilution over night at 4 °C can also help to produce a robust and specific signal in many cases.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-07-09

What application is the blocking peptide sc-393438 P appropriate for?

Preguntado por: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-28
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Rated 5 de 5 por de Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Fecha de publicación: 2015-02-23
Rated 5 de 5 por de Nice Western Blot data of UDP Nice Western Blot data of UDP-GlcDH expression in Hep G2, HeLa and A-431 whole cell lysates and human liver tissue extract . -SCBT QC
Fecha de publicación: 2014-04-23
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