Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TSG-6 CRISPR/Cas9 KO Plasmid (m): sc-423437
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSG-6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TSG-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TSG-6 Antibody (A38.1.20): sc-65886
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSG-6 CRISPR/Cas9 KO Plasmid (m) | sc-423437 | 20 µg | $397.00 |
Overview
Tnfaip6 encodes tumor necrosis factor‑α–induced protein 6 (TSG‑6), a secreted hyaluronan-binding protein that remodels the extracellular matrix during inflammation and tissue injury. TSG‑6 catalyzes transfer of heavy chains from inter‑α‑inhibitor to hyaluronan, promoting formation of stable HA-rich matrices that influence leukocyte adhesion, cell migration, and resolution of inflammatory responses. In mouse systems, TNFAIP6 is rapidly induced downstream of pro-inflammatory cues such as TNF/NF‑κB and modulates chemokine activity, protease networks, and matrix organization. Dysregulated TSG‑6/HA biology has been linked to models of arthritis, lung and intestinal inflammation, wound repair, and fibrosis, supporting its use as a mechanistic node connecting cytokine signaling to ECM dynamics.
TSG-6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnfaip6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnfaip6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnfaip6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TSG-6 protein expression.
This CRISPR knockout system enables efficient generation of Tnfaip6-deficient cell models for investigation of TSG-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnfaip6 exon(s) critical for TSG-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnfaip6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TSG-6 CRISPR/Cas9 KO Plasmid (m) and TSG-6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnfaip6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TSG-6 HDR Plasmid (m) and TSG-6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnfaip6 homology arms to support homology-directed repair at defined Tnfaip6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.