Molecular structure of Tris base, CAS Number: 77-86-1
Tris base (CAS 77-86-1)
See product citations (13)
Alternate Names:
Tris base is also known as Tris(hydroxymethyl)aminomethane.
Application:
Tris base is widely used as a biological buffer or as a component of buffer formulations. Tris base has buffering capabilities in the pH range of 7.0-9.0.
CAS Number:
77-86-1
Purity:
≥99%
Molecular Weight:
121.14
Molecular Formula:
C4H11NO3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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Description
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Safety Information
SDS & Certificate of Analysis
Tris base is widely used as a biological buffer or as a component of buffer formulations such as TAE (sc-296645) and TBE (sc-296650) buffers. It has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris base has been reported to interfere with the activity of a number of enzymes, therefore it should be used carefully when studying proteins. Tris base is also known as THAM, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, and Tris(hydroxymethyl)aminomethane.
Tris base (CAS 77-86-1) References
- The treatment of acidosis in acute lung injury with tris-hydroxymethyl aminomethane (THAM). | Kallet, RH., et al. 2000. Am J Respir Crit Care Med. 161: 1149-53. PMID: 10764304
- The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition. | Desmarais, WT., et al. 2002. Structure. 10: 1063-72. PMID: 12176384
- Binding of Tris to Bacillus licheniformis alpha-amylase can affect its starch hydrolysis activity. | Ghalanbor, Z., et al. 2008. Protein Pept Lett. 15: 212-4. PMID: 18289113
- Conformational Dynamics of DNA G-Quadruplex in Solution Studied by Kinetic Capillary Electrophoresis Coupled On-line with Mass Spectrometry. | Mironov, GG., et al. 2014. ChemistryOpen. 3: 58-64. PMID: 24808992
- Effect of TRIS and bicarbonate as buffers on anesthetic efficacy of tricaine methane sulfonate in Zebrafish (Danio rerio). | Spears, J., et al. 2014. Zebrafish. 11: 590-6. PMID: 25264822
- In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver. | Ding, W., et al. 2016. J Vis Exp.. PMID: 27166647
- Mitigating the Effects of Electrode Biofouling-Induced Impedance for Improved Long-Term Electrochemical Measurements In Vivo. | Seaton, BT., et al. 2020. Anal Chem. 92: 6334-6340. PMID: 32298105
- Investigation of Break-Induced Replication in Yeast. | Osia, B., et al. 2021. Methods Mol Biol. 2153: 307-328. PMID: 32840789
- FTIR Investigation of Secondary Structure of Reteplase Inclusion Bodies Produced in Escherichia coli in Terms of Urea Concentration. | Naeimipour, S., et al. 2020. Iran J Pharm Res. 19: 175-181. PMID: 33224222
- An optimized two-step chromatin immunoprecipitation protocol to quantify the associations of two separate proteins and their common target DNA. | He, L., et al. 2021. STAR Protoc. 2: 100504. PMID: 33997818
- Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells. | Holliday, H., et al. 2021. STAR Protoc. 2: 100514. PMID: 34013210
- Surface biotinylation of primary neurons to monitor changes in AMPA receptor surface expression in response to kainate receptor stimulation. | Nair, JD., et al. 2021. STAR Protoc. 2: 100992. PMID: 34934960
- A two-phase protocol for ambient hydrogen production using Chlamydomonas reinhardtii. | Elman, T. and Yacoby, I. 2022. STAR Protoc. 3: 101640. PMID: 36042878
- Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy. | Penumutchu, S., et al. 2022. STAR Protoc. 3: 101842. PMID: 36595882
- A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection. | Gong, XY. and Zhang, YB. 2022. STAR Protoc. 3: 101844. PMID: 36595883
Activator of:
anti-Cy5.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tris base, 500 g | sc-3715 | 500 g | $34.00 | |||
Tris base, 1 kg | sc-3715A | 1 kg | $63.00 | |||
Tris base, 5 kg | sc-3715B | 5 kg | $315.00 | |||
Tris base, 10 kg | sc-3715C | 10 kg | $630.00 |