TLK1 Double Nickase Plasmid (h): sc-407634-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TLK1 Double Nickase Plasmid (h) and TLK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TLK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLK1 Antibody (G-8): sc-393515
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLK1 Double Nickase Plasmid (h) | sc-407634-NIC | 20 µg | $410.00 | |||
TLK1 Double Nickase Plasmid (h2) | sc-407634-NIC-2 | 20 µg | $410.00 |
TLK1 (Tousled-like kinase 1) encodes a serine/threonine kinase that coordinates DNA replication with chromatin dynamics and genome maintenance. TLK1 phosphorylates chromatin-associated substrates such as ASF1 to regulate nucleosome assembly, replication-coupled histone deposition, and recovery from replication stress. Through these activities, TLK1 interfaces with cell-cycle checkpoint control and DNA damage response pathways that preserve chromosome integrity. Dysregulated TLK1 activity has been investigated in the context of aberrant proliferation and genome instability phenotypes relevant to cancer biology and stress-response signaling.
TLK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TLK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TLK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TLK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TLK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.