TCTE1 CRISPR/Cas9 KO Plasmid (m): sc-423315
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TCTE1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TCTE1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCTE1 CRISPR/Cas9 KO Plasmid (m) | sc-423315 | 20 µg | $397.00 |
Overview
Tcte1 encodes TCTE1, a cilia- and flagella-associated protein implicated in the structural integrity and motility of the axoneme. In mouse, TCTE1 is linked to processes governing microtubule organization within the ciliary compartment, supporting coordinated dynein-driven beating and efficient intraflagellar transport. Perturbation of axonemal components can disrupt ciliogenesis and sperm flagellar function, providing mechanistic connections to male infertility phenotypes and broader ciliopathy-related pathways. As a ciliary module, TCTE1 is relevant for studying how axonemal architecture interfaces with cell signaling programs that depend on intact primary and motile cilia.
TCTE1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tcte1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tcte1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tcte1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TCTE1 protein expression.
This CRISPR knockout system enables efficient generation of Tcte1-deficient cell models for investigation of TCTE1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tcte1 exon(s) critical for TCTE1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tcte1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TCTE1 CRISPR/Cas9 KO Plasmid (m) and TCTE1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tcte1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TCTE1 HDR Plasmid (m) and TCTE1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tcte1 homology arms to support homology-directed repair at defined Tcte1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.