Stra6 CRISPR/Cas9 KO Plasmid (h): sc-405364
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Stra6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Stra6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stra6 CRISPR/Cas9 KO Plasmid (h) | sc-405364 | 20 µg | $397.00 |
Overview
STRA6 encodes Stra6, a multi-pass membrane receptor that mediates cellular uptake of vitamin A by binding retinol–retinol binding protein (RBP) complexes at the cell surface. By controlling intracellular retinoid availability, Stra6 influences retinoic acid signaling and downstream transcriptional programs that regulate differentiation, epithelial homeostasis, and developmental patterning. STRA6 activity intersects with retinoid metabolism networks involving LRAT, RDH/ALDH enzymes, and nuclear retinoic acid receptors, thereby shaping cell fate decisions and tissue integrity. Dysregulation or genetic variation in STRA6 has been linked to developmental abnormalities and vision-related phenotypes consistent with perturbed retinoid transport and signaling.
Stra6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STRA6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STRA6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STRA6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Stra6 protein expression.
This CRISPR knockout system enables efficient generation of STRA6-deficient cell models for investigation of Stra6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STRA6 exon(s) critical for Stra6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STRA6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Stra6 CRISPR/Cas9 KO Plasmid (h) and Stra6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STRA6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Stra6 HDR Plasmid (h) and Stra6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STRA6 homology arms to support homology-directed repair at defined STRA6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.