SPRN CRISPR/Cas9 KO Plasmid (h): sc-407259
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPRN CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPRN genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPRN CRISPR/Cas9 KO Plasmid (h) | sc-407259 | 20 µg | $397.00 |
Overview
SPRN encodes the shadow of prion protein (Shadoo), a glycosylphosphatidylinositol (GPI)-anchored membrane protein enriched in the nervous system and implicated in prion-related biology. Shadoo shares structural and functional features with PRNP family proteins and is linked to processes such as protein quality control, membrane microdomain organization, and cellular stress responses. Altered SPRN expression has been associated with prion disease susceptibility and neurodegenerative phenotypes in experimental models, supporting its relevance to pathways governing proteostasis and neuronal viability. Investigating SPRN helps clarify how prion-like protein networks influence synaptic maintenance and responses to misfolded protein stress.
SPRN CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPRN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPRN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPRN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPRN protein expression.
This CRISPR knockout system enables efficient generation of SPRN-deficient cell models for investigation of SPRN signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPRN exon(s) critical for SPRN function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPRN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPRN CRISPR/Cas9 KO Plasmid (h) and SPRN CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPRN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPRN HDR Plasmid (h) and SPRN HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPRN homology arms to support homology-directed repair at defined SPRN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.