SPARC Double Nickase Plasmid (m): sc-423101-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPARC Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SPARC Double Nickase Plasmid (m) and SPARC Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sparc. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPARC Antibody (AON-1): sc-33645
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPARC Double Nickase Plasmid (m) | sc-423101-NIC | 20 µg | $410.00 | |||
SPARC Double Nickase Plasmid (m2) | sc-423101-NIC-2 | 20 µg | $410.00 |
Sparc encodes SPARC (secreted protein acidic and rich in cysteine), a matricellular glycoprotein that modulates extracellular matrix assembly and cell–matrix interactions rather than serving as a structural ECM component. In mouse tissues, SPARC influences collagen fibrillogenesis, focal adhesion dynamics, and growth factor bioavailability, integrating with pathways that regulate cell migration, proliferation, and tissue remodeling. SPARC activity is frequently linked to wound repair, angiogenic signaling contexts, and fibrotic remodeling programs through effects on stromal organization and matrix stiffness. Altered SPARC expression has been associated with changes in tumor microenvironment behavior, inflammatory remodeling, and organ fibrosis phenotypes in experimental models, making it a useful node for studying ECM-driven disease mechanisms.
SPARC Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sparc locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sparc. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sparc function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sparc-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.