Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SPACR CRISPR/Cas9 KO Plasmid (h): sc-406681
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPACR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPACR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPACR Antibody (G-11): sc-377366
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPACR CRISPR/Cas9 KO Plasmid (h) | sc-406681 | 20 µg | $397.00 |
Overview
IMPG1 encodes SPACR, a large secreted proteoglycan that is a key structural component of the retinal interphotoreceptor matrix and contributes to adhesion and spacing between photoreceptor outer segments and the retinal pigment epithelium. Through extensive glycosylation and interactions with other extracellular matrix constituents, SPACR supports matrix organization, diffusion of signaling molecules, and maintenance of photoreceptor homeostasis. Disruption of IMPG1 is associated with inherited retinal disorders, including macular dystrophies, highlighting its relevance to studies of extracellular matrix integrity and photoreceptor survival pathways. As a retina-enriched matrix protein, SPACR is also used to investigate how pericellular matrices modulate cell–cell communication and stress responses in the neuroepithelium.
SPACR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IMPG1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IMPG1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IMPG1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPACR protein expression.
This CRISPR knockout system enables efficient generation of IMPG1-deficient cell models for investigation of SPACR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IMPG1 exon(s) critical for SPACR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IMPG1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPACR CRISPR/Cas9 KO Plasmid (h) and SPACR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IMPG1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPACR HDR Plasmid (h) and SPACR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IMPG1 homology arms to support homology-directed repair at defined IMPG1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.