Sox-4 Lentiviral Activation Particles (h): sc-401217-LAC

Human SOX4 encodes the transcription factor Sox-4, a member of the SRY-related HMG-box family that binds DNA and coordinates context-dependent gene regulatory programs during development and tissue homeostasis. Sox-4 activity influences cell fate specification, epithelial–mesenchymal transition-associated transcriptional networks, and differentiation processes, interfacing with signaling pathways such as Wnt/β-catenin, TGF-β, and Notch. Dysregulated SOX4 expression has been linked to altered proliferation, stem-like states, and invasion-associated phenotypes across multiple cancer and hematologic disease contexts, and it is also studied in neurodevelopmental and immune-related biology. As a nuclear regulator with broad downstream targets, SOX4 provides a tractable node for dissecting transcriptional circuitry, chromatin-state dependencies, and lineage plasticity.

Sox-4 Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient SOX4 upregulation across a broader range of human cell types.

Sox-4 Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the SOX4 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Sox-4 expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native SOX4 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.