Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNAP47 CRISPR/Cas9 KO Plasmid (h): sc-410264
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNAP47 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SNAP47 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNAP47 Antibody (D-11): sc-514428
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAP47 CRISPR/Cas9 KO Plasmid (h) | sc-410264 | 20 µg | $397.00 |
Overview
SNAP47 encodes a SNAP receptor (SNARE) family protein that participates in vesicle docking and membrane fusion by engaging cognate syntaxins and VAMPs to regulate exocytosis and endomembrane trafficking. It has been implicated in specialized secretory pathways, including neurotransmitter release and regulated cargo delivery, linking SNAP47 activity to synaptic vesicle cycling, membrane recycling, and spatial control of protein localization. Through its role in SNARE complex dynamics, SNAP47 influences cellular processes such as neurite outgrowth, receptor trafficking, and stimulus-coupled secretion. Dysregulated vesicle trafficking and synaptic signaling are relevant to neurodevelopmental and neurodegenerative research contexts, making SNAP47 a useful target for interrogating transport-dependent phenotypes.
SNAP47 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SNAP47 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SNAP47 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SNAP47 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SNAP47 protein expression.
This CRISPR knockout system enables efficient generation of SNAP47-deficient cell models for investigation of SNAP47 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SNAP47 exon(s) critical for SNAP47 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SNAP47 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SNAP47 CRISPR/Cas9 KO Plasmid (h) and SNAP47 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SNAP47 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SNAP47 HDR Plasmid (h) and SNAP47 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SNAP47 homology arms to support homology-directed repair at defined SNAP47 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.