Sec5 Double Nickase Plasmid (h): sc-405088-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sec5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sec5 Double Nickase Plasmid (h) and Sec5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EXOC2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sec5 Antibody (F-7): sc-393230
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec5 Double Nickase Plasmid (h) | sc-405088-NIC | 20 µg | $410.00 | |||
Sec5 Double Nickase Plasmid (h2) | sc-405088-NIC-2 | 20 µg | $410.00 |
Human EXOC2 encodes Sec5, an essential subunit of the octameric exocyst tethering complex that spatially coordinates secretory vesicle docking and fusion at the plasma membrane. Sec5 integrates signals from small GTPases, including RalA/RalB, to couple vesicle trafficking with polarized exocytosis, membrane remodeling, and targeted delivery of receptors and adhesion molecules. Through these functions, EXOC2 contributes to processes such as cell migration, cytokinesis, and maintenance of epithelial polarity, and its dysregulation has been associated with altered signaling outputs linked to oncogenic transformation and invasive behavior. Sec5 has also been used to probe how exocyst-dependent trafficking shapes receptor recycling and pathway organization in diverse human cell types.
Sec5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EXOC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EXOC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EXOC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EXOC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.