Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SAP 18 Double Nickase Plasmid (h): sc-404214-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAP 18 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SAP 18 Double Nickase Plasmid (h) and SAP 18 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SAP18. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAP 18 Antibody (C-3): sc-365377
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP 18 Double Nickase Plasmid (h) | sc-404214-NIC | 20 µg | $410.00 | |||
SAP 18 Double Nickase Plasmid (h2) | sc-404214-NIC-2 | 20 µg | $410.00 |
Human SAP18 encodes SAP 18, a core component of the Sin3A/HDAC transcriptional repression machinery that helps couple sequence-specific DNA-binding factors to histone deacetylation and chromatin compaction. Through its participation in HDAC-dependent gene silencing, SAP 18 influences transcriptional programs governing cell-cycle control, differentiation, and cellular stress responses. SAP18-associated complexes also intersect with RNA processing and apoptotic signaling pathways, reflecting broad roles in maintaining nuclear homeostasis. Altered regulation of Sin3/HDAC components, including SAP18, has been linked to dysregulated epigenetic states observed in cancer and other disorders characterized by aberrant transcriptional control.
SAP 18 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SAP18 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SAP18. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SAP18 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SAP18-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.