RhoGEF p115 Antibody (G-12): sc-74564

RhoGEF p115 Antibody (G-12) is a mouse monoclonal IgG1 antibody that detects RhoGEF p115 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RhoGEF p115 plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor (GEF) specifically for Rho GTPases, which are pivotal in regulating various cellular processes, including cytoskeletal dynamics and cell migration. Anti-RhoGEF p115 antibody (G-12) selectively recognizes RhoGEF p115′s activation of Rho GTPases, while not affecting Rac or Cdc42, underscoring RhoGEF p115′s importance in maintaining the balance of signaling pathways that govern cell shape and movement. This specificity is vital for processes such as wound healing and tissue development, where precise control of the cytoskeleton is necessary. RhoGEF p115 is localized in the cytoplasm, where RhoGEF p115 interacts with other signaling molecules, further emphasizing RhoGEF p115′s role in orchestrating complex cellular responses. RhoGEF p115 (G-12) antibody is an essential tool for researchers studying the intricate mechanisms of cell signaling and the functional implications of Rho GTPase activation in health and disease.

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RhoGEF p115 Antibody (G-12) References:

1. Functional interaction between the cytoplasmic leucine-zipper domain of HIV-1 gp41 and p115-RhoGEF.  |  Zhang, H., et al. 1999. Curr Biol. 9: 1271-4. PMID: 10556093
2. Galpha 13 requires palmitoylation for plasma membrane localization, Rho-dependent signaling, and promotion of p115-RhoGEF membrane binding.  |  Bhattacharyya, R. and Wedegaertner, PB. 2000. J Biol Chem. 275: 14992-9. PMID: 10747909
3. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor.  |  García-Mata, R. and Sztul, E. 2003. EMBO Rep. 4: 320-5. PMID: 12634853
4. Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment.  |  Bhattacharyya, R. and Wedegaertner, PB. 2003. FEBS Lett. 540: 211-6. PMID: 12681510
5. Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner.  |  Diao, A., et al. 2008. J Biol Chem. 283: 6957-67. PMID: 18167358
6. P115 RhoGEF and microtubules decide the direction apoptotic cells extrude from an epithelium.  |  Slattum, G., et al. 2009. J Cell Biol. 186: 693-702. PMID: 19720875
7. Mechanistic insights into specificity, activity, and regulatory elements of the regulator of G-protein signaling (RGS)-containing Rho-specific guanine nucleotide exchange factors (GEFs) p115, PDZ-RhoGEF (PRG), and leukemia-associated RhoGEF (LARG).  |  Jaiswal, M., et al. 2011. J Biol Chem. 286: 18202-12. PMID: 21454492
8. PRL-1 protein promotes ERK1/2 and RhoA protein activation through a non-canonical interaction with the Src homology 3 domain of p115 Rho GTPase-activating protein.  |  Bai, Y., et al. 2011. J Biol Chem. 286: 42316-42324. PMID: 22009749
9. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF.  |  Scott, DW., et al. 2016. Mol Biol Cell. 27: 1420-30. PMID: 26985018
10. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation.  |  Singh, NK., et al. 2017. J Biol Chem. 292: 14080-14091. PMID: 28655771

The preferred RhoGEF p115 antibody is RhoGEF p115 Antibody (C-9): sc-74565.