RhoGEF p115 Antibody (D-11): sc-166341
- RhoGEF p115 Antibody (D-11) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 48-75 near the N-terminus of RhoGEF p115 of human origin
- recommended for detection of RhoGEF p115 of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RhoGEF p115 Antibody (D-11). This work is in progress.
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RhoGEF p115 Antibody (D-11) is a mouse monoclonal IgG2a antibody that detects RhoGEF p115 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RhoGEF p115 plays a crucial role in cellular signaling by acting as a guanine nucleotide exchange factor (GEF) specifically for Rho family GTPases, which are essential for regulating various cellular processes, including cytoskeletal dynamics and cell migration. RhoGEF p115′s ability to selectively activate Rho GTPases, while not affecting Rac or Cdc42, highlights RhoGEF p115′s importance in maintaining the specificity of signaling pathways that govern cell shape and movement. This specificity is vital for processes such as wound healing and immune responses, where precise control of cell behavior is necessary. RhoGEF p115 is predominantly located in the cytoplasm, where RhoGEF p115 interacts with other signaling molecules to facilitate the exchange of GDP for GTP, thus toggling the Rho GTPases between their active and inactive states. The regulation of these GTPases by RhoGEF p115 underscores RhoGEF p115′s significance in the broader context of cellular communication and function, making RhoGEF p115 a valuable target for research in cell biology and related fields.
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RhoGEF p115 Antibody (D-11) References:
- Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment. | Bhattacharyya, R. and Wedegaertner, PB. 2003. FEBS Lett. 540: 211-6. PMID: 12681510
- Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner. | Diao, A., et al. 2008. J Biol Chem. 283: 6957-67. PMID: 18167358
- Reversible translocation of p115-RhoGEF by G(12/13)-coupled receptors. | Meyer, BH., et al. 2008. J Cell Biochem. 104: 1660-70. PMID: 18320579
- Differences in Galpha12- and Galpha13-mediated plasma membrane recruitment of p115-RhoGEF. | Bhattacharyya, R., et al. 2009. Cell Signal. 21: 996-1006. PMID: 19249348
- P115 RhoGEF and microtubules decide the direction apoptotic cells extrude from an epithelium. | Slattum, G., et al. 2009. J Cell Biol. 186: 693-702. PMID: 19720875
- Modulation of a GEF switch: autoinhibition of the intrinsic guanine nucleotide exchange activity of p115-RhoGEF. | Chen, Z., et al. 2011. Protein Sci. 20: 107-17. PMID: 21064165
- PRL-1 protein promotes ERK1/2 and RhoA protein activation through a non-canonical interaction with the Src homology 3 domain of p115 Rho GTPase-activating protein. | Bai, Y., et al. 2011. J Biol Chem. 286: 42316-42324. PMID: 22009749
- Activation of p115-RhoGEF requires direct association of Gα13 and the Dbl homology domain. | Chen, Z., et al. 2012. J Biol Chem. 287: 25490-500. PMID: 22661716
- Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. | Scott, DW., et al. 2016. Mol Biol Cell. 27: 1420-30. PMID: 26985018
- p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. | Kozasa, T., et al. 1998. Science. 280: 2109-11. PMID: 9641915
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RhoGEF p115 Antibody (D-11) | sc-166341 | 200 µg/ml | $322.00 | |||
RhoGEF p115 (D-11) Neutralizing Peptide | sc-166341 P | 100 µg/0.5 ml | $69.00 |