Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PP2A-Cβ CRISPR/Cas9 KO Plasmid (h): sc-400937
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP2A-Cβ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PP2A-Cβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PP2A-Cα/β Antibody (1D6): sc-80665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP2A-Cβ CRISPR/Cas9 KO Plasmid (h) | sc-400937 | 20 µg | $397.00 |
Overview
PPP2CB encodes the catalytic beta subunit of protein phosphatase 2A (PP2A-Cβ), a major serine/threonine phosphatase that forms heterotrimeric PP2A holoenzymes with diverse regulatory B subunits to control substrate specificity and subcellular localization. PP2A-Cβ counterbalances kinase signaling by dephosphorylating key nodes in pathways governing cell-cycle progression, DNA damage responses, and stress signaling, including AKT/MAPK and multiple mitotic regulators. Through these activities, PP2A helps maintain phosphorylation homeostasis affecting transcriptional programs, cytoskeletal dynamics, and metabolic control. Dysregulated PP2A signaling and altered PPP2CB expression or regulation have been linked to aberrant proliferation and survival signaling, with relevance to oncology and neurobiology-focused studies of phosphatase-dependent signaling networks.
PP2A-Cβ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PPP2CB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PPP2CB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PPP2CB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PP2A-Cβ protein expression.
This CRISPR knockout system enables efficient generation of PPP2CB-deficient cell models for investigation of PP2A-Cβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PPP2CB exon(s) critical for PP2A-Cβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PPP2CB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PP2A-Cβ CRISPR/Cas9 KO Plasmid (h) and PP2A-Cβ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PPP2CB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PP2A-Cβ HDR Plasmid (h) and PP2A-Cβ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PPP2CB homology arms to support homology-directed repair at defined PPP2CB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.