PP2A-B56-beta Anticuerpo (D-10) recomendado para detectarPP2A-B56-β de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

PP2A-B56-β Anticuerpo (D-10): sc-515681

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Ficha técnica

    • PP2A-B56-β Anticuerpo (D-10) es un monoclonal de ratón IgM (kappa light chain) proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 451-472 cerca del C-terminus de oe orgiendf humanorigin
    • recomendado para detectarPP2A-B56-β de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for PP2A-B56-β Antibody (D-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with PP2A-B56-β Antibody (D-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
loading

How much sample do you recommend loading on the gel for Western blot detection using PP2A-B56-β (D-10): sc-515681?

Preguntado por: jenniferc
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-03-01
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