Anti-POGK Anticuerpo F-1 es un monoclonal de ratón IgG1 (kappa light chain) POGK Anticuerpo proporcionado como 200 µg/ml
Producido contra los amino ácidos 108-407 localizado dentro de un dominio interno de POGK de origen human
POGK Anticuerpo (F-1) es recomendado para detectar POGK de human origen, mediante WB, IP, IF y ELISA
POGK Anticuerpo (F-1) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de POGK (F-1): sc-515752.
m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for POGK Antibody (F-1) for WB applications. These reagents are now offered in bundles with POGK Antibody (F-1) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
POGK Antibody (F-1) is a high quality monoclonal POGK antibody (also designated POGK antibody) suitable for the detection of the POGK protein of human origin. POGK Antibody (F-1) is available as both the non-conjugated anti-POGK antibody form, as well as multiple conjugated forms of anti-POGK antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. POGK (pogo transposable element with KRAB domain), also known as BASS2 or LST003, is a 609 amino acid protein that may be a member of the DNA-mediated transposon family. Localizing to the nucleus, POGK contains an N-terminal KRAB (Kruppel-associated box) domain, an HTH CENP-B-type (helix-turn-helix and centromere protein B-type) DNA-binding domain and a C-terminal DDE domain. The KRAB domain is a potent transcriptional repression motif and is approximately 75 amino acids in length. DDE domains are catalytic motifs consisting of two conserved aspartic acids separated by 70 amino acids and a conserved glutamic acid that is further separated by another 35 amino acids. DDE domains are characteristic of DNA transposases and retroviral integrases.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How much sample do you recommend loading on the gel for Western blot detection using POGK (F-1): sc-515752?
Preguntado por: Trav11
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
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