Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PLAA CRISPR/Cas9 KO Plasmid (m): sc-422286
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLAA CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLAA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PLAA Antibody (E-1): sc-390454
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLAA CRISPR/Cas9 KO Plasmid (m) | sc-422286 | 20 µg | $397.00 |
Overview
Plaa encodes phospholipase A2-activating protein (PLAA), a conserved cytosolic factor implicated in phospholipid signaling and membrane dynamics through regulation of phospholipase A2 activity and arachidonic acid–derived lipid mediator production. PLAA contains ubiquitin-binding domains and has been linked to protein quality control processes, including ubiquitin-dependent trafficking and turnover of membrane-associated proteins. Through these functions, PLAA can influence inflammatory signaling cascades, endolysosomal organization, and stress-responsive pathways that intersect with innate immunity and cellular homeostasis. Dysregulation of lipid signaling and ubiquitin-mediated proteostasis is broadly relevant to neuroinflammation, metabolic phenotypes, and degenerative processes, making Plaa a useful node for mechanistic studies in mouse models.
PLAA CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Plaa gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Plaa together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Plaa open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLAA protein expression.
This CRISPR knockout system enables efficient generation of Plaa-deficient cell models for investigation of PLAA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Plaa exon(s) critical for PLAA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Plaa genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLAA CRISPR/Cas9 KO Plasmid (m) and PLAA CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Plaa locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLAA HDR Plasmid (m) and PLAA HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Plaa homology arms to support homology-directed repair at defined Plaa target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.