PLA2R CRISPR/Cas9 KO Plasmid (m): sc-422280
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLA2R CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PLA2R genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLA2R CRISPR/Cas9 KO Plasmid (m) | sc-422280 | 20 µg | $397.00 |
Overview
Pla2r1 encodes PLA2R, a type I transmembrane C-type lectin receptor that binds secreted phospholipase A2 enzymes and contributes to extracellular ligand capture and receptor-mediated endocytosis. Through regulating ligand internalization and membrane-associated signaling cues, PLA2R influences lipid mediator availability, inflammatory tone, and cell–matrix interactions in a context-dependent manner. In mouse tissues, Pla2r1 expression has been linked to immune and stromal biology, with reported relevance to inflammatory processes and fibrotic remodeling. Altered PLA2R pathway activity is frequently investigated in models of organ injury and immune-driven tissue responses, where receptor–ligand dynamics can shape downstream transcriptional and metabolic programs.
PLA2R CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pla2r1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pla2r1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pla2r1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PLA2R protein expression.
This CRISPR knockout system enables efficient generation of Pla2r1-deficient cell models for investigation of PLA2R signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pla2r1 exon(s) critical for PLA2R function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pla2r1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PLA2R CRISPR/Cas9 KO Plasmid (m) and PLA2R CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pla2r1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PLA2R HDR Plasmid (m) and PLA2R HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pla2r1 homology arms to support homology-directed repair at defined Pla2r1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.