Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 4-kinase II α CRISPR/Cas9 KO Plasmid (h): sc-405423
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 4-kinase II α CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PI 4-kinase II α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 4-kinase II α Antibody (B-5): sc-390026
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 4-kinase II α CRISPR/Cas9 KO Plasmid (h) | sc-405423 | 20 µg | $397.00 |
Overview
PI4K2A encodes phosphatidylinositol 4-kinase II alpha (PI 4-kinase II alpha), a membrane-associated lipid kinase that generates phosphatidylinositol 4-phosphate (PI4P) to support phosphoinositide signaling and membrane identity. PI4P produced by PI4K2A contributes to Golgi and endosomal trafficking, clathrin-mediated sorting, and downstream phosphoinositide conversion that influences receptor transport and signaling dynamics. Through these roles in vesicle biogenesis and membrane remodeling, PI4K2A is relevant to studies of endomembrane organization, nutrient sensing, and stress responses. Altered phosphoinositide metabolism and trafficking programs linked to PI4K2A have been associated with mechanisms implicated in cancer biology, neurobiology, and host–pathogen interactions.
PI 4-kinase II α CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PI4K2A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PI4K2A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PI4K2A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PI 4-kinase II α protein expression.
This CRISPR knockout system enables efficient generation of PI4K2A-deficient cell models for investigation of PI 4-kinase II α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PI4K2A exon(s) critical for PI 4-kinase II α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PI4K2A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PI 4-kinase II α CRISPR/Cas9 KO Plasmid (h) and PI 4-kinase II α CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PI4K2A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PI 4-kinase II α HDR Plasmid (h) and PI 4-kinase II α HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PI4K2A homology arms to support homology-directed repair at defined PI4K2A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.