Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PI 3-kinase p101 Antibody (E-12) is a high quality monoclonal PI 3-kinase p101 antibody (also designated PI3K antibody, p101 antibody, PIK3R5 antibody or phosphoinositide-3-kinase regulatory subunit 5 antibody) suitable for the detection of the PI 3-kinase p101 protein of human origin. PI 3-kinase p101 Antibody (E-12) is available as both the non-conjugated anti-PI 3-kinase p101 antibody form, as well as multiple conjugated forms of anti-PI 3-kinase p101 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. PI 3-kinase p101 is an 880 amino acid protein that acts as a regulatory subunit of the PI3 kinase γ complex. PI 3-kinase p101 interacts with PI 3-kinase p110γ to form the PI3 kinase γ complex, which is activated by Gβγ proteins and plays a role in many physiological processes, such as cardiac function, neutrophil chemotaxis and mast cell degranulation. Specifically, the PI3 kinase γ complex is involved in suppression of apoptosis, cellular transport and cell motility. Binding of the PI 3-kinase p110γ subunit to PI 3-kinase p101 is dependent on the N-terminal region of PI 3-kinase p101. With highest expression in leukocytes, spleen lymph node thymus and bone marrow, PI 3-kinase p101 is subcellularly located in the nucleus, cytoplasm, or it can exist as a peripheral membrane protein. There are two isoforms of PI 3-kinase p101 that are produced as a result of alternative splicing.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IS THERE AN OPTIMAL DILUTION OF THIS ANTIBODY FOR IMMUNOHISTOCHEMISTRY? IS NECESARY ANY PRETRATMENT PREVIOUS?
Preguntado por: HORACIO
Thank you for your question. The recommended starting dilution for IHC for this antibody is 1:50. We do recommend doing antigen retrieval. Here is a link to the recommended protocol:
Respondida por: Tech Service
Fecha de publicación: 2020-02-24
For Western Blot, is it recommended to use denatured or non-denatured conditions with PI 3-kinase p101 (E-12): sc-390916 monoclonal antibody?
Preguntado por: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn