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western blotting image from recommended PGC1a (D-5) monoclonal antibody replacement. Image 390096 — Este anticuerpo policlonal ha dejado de fabricarse. Consulta nuestro anticuerpo monoclonal recomendado PGC1a (D-5) (se muestran datos de muestra).

Este anticuerpo policlonal ha dejado de fabricarse. Consulta nuestro anticuerpo monoclonal recomendado PGC1a (D-5) (se muestran datos de muestra).

PGC-1α Anticuerpo (H-300): sc-13067

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PGC-1 alpha Anticuerpo H-300es un policlonal de conejo IgG proporcionado como 200 µg/ml
epítopo correspondiente a los amino ácidos 1-300 localizados en el N-terminus of PGC-1 de origen human
Anticuerpo policlonal discontinuado

Ver las publicaciones del producto (192)

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Más PGC Anticuerpos incluyendo PGC-1 α y PGC-1 β

Información sobre pedidos

PGC1a (D-5): sc-518025 [ Monoclonal recomendado como alternativa a PGC-1α (H-300) ]

Nombre del producto	Número de catálogo	UNIDAD	Precio	CANTIDAD	Favoritos
PGC1a Anticuerpo (D-5)	sc-518025	200 µg/ml	$316.00
Paquete de PGC1a (D-5): m-IgG Fc BP-HRP	sc-531597	200 µg Ab; 10 µg BP	$354.00
Paquete de PGC1a (D-5): m-IgGκ BP-HRP	sc-525331	200 µg Ab, 40 µg BP	$354.00
Paquete de PGC1a (D-5): m-IgG_2a BP-HRP	sc-547825	200 µg Ab; 10 µg BP	$354.00
PGC1a Anticuerpo (D-5) AC	sc-518025 AC	500 µg/ml, 25% agarose	$416.00
PGC1a Anticuerpo (D-5) HRP	sc-518025 HRP	200 µg/ml	$316.00
PGC1a Anticuerpo (D-5) FITC	sc-518025 FITC	200 µg/ml	$330.00
PGC1a Anticuerpo (D-5) PE	sc-518025 PE	200 µg/ml	$343.00
PGC1a Anticuerpo (D-5) Alexa Fluor^® 488	sc-518025 AF488	200 µg/ml	$357.00
PGC1a Anticuerpo (D-5) Alexa Fluor^® 546	sc-518025 AF546	200 µg/ml	$357.00
PGC1a Anticuerpo (D-5) Alexa Fluor^® 594	sc-518025 AF594	200 µg/ml	$357.00
PGC1a Anticuerpo (D-5) Alexa Fluor^® 647	sc-518025 AF647	200 µg/ml	$357.00
PGC1a Anticuerpo (D-5) Alexa Fluor^® 680	sc-518025 AF680	200 µg/ml	$357.00
PGC1a Anticuerpo (D-5) Alexa Fluor^® 790	sc-518025 AF790	200 µg/ml	$357.00

If the product is specified for mice, can it work on rat tissue¿

Preguntado por: XimenaYri

Thank you for your question. PGC-1α(H-300) was recommended for detection of PGC-1α of mouse, rat and human origin. This antibody has been discontinued. We recommend you use one of our monoclonal alternatives, such as PGC-1α Anticuerpo (D-5): sc-518025, which is recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.

Respondida por: Tech Support Europe

Fecha de publicación: 2021-11-09

what is the working concentration to be used

Preguntado por: Thara

Thank you for your question. Please refer to our protocols for the recommended concentrations for our antibodies. If you have any further questions, please contact Technical Service. https://www.scbt.com/resources/protocols

Respondida por: Technical Service 3

Fecha de publicación: 2020-11-25

I am getting a band between 37 and 50 kDa with this antibody. Has someone else reported this?

Preguntado por: Kranti

Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa, but do not have information regarding a smaller size. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.

Respondida por: Technical Support

Fecha de publicación: 2020-11-02

What is the expected molecular weight for bands?

Preguntado por: JJJJ55

Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.

Respondida por: SCBT Heidelberg Support

Fecha de publicación: 2019-03-07

I performed an immunoprecipitation using this antibody (PGC-1 alpha Antibody - H-300) and I had a problem since the heavy chain (HC, 55 kDa) of the IP antibody were also recognized.

Preguntado por: johnny86

Thank you for your question. This is rabbit polyclonal antibody, PGC-1α (H-300): sc-13067, is expected to detect the PGC-1α protein at approximately 91 kDa. The heavy chain of IgG is approximately 55 kDa. Therefore, observing the heavy chain IgG band from your immunoprecipitating antibody should not interfere with detection of your target protein. However, if you wish to not observe the heavy chain, then we would recommend using a different antibody for your Western blot. For example, a mouse monoclonal against PGC-1α. Then you could use an anti-mouse IgG secondary antibody, that would not recognize the heavy chain of the rabbit IgG primary used for immunoprecipitation.

Respondida por: Technical Support

Fecha de publicación: 2018-08-17

I used this antibody on cells and get 2 bands very close together which one is pgc1 alpha upper or lower

Preguntado por: Joey88

Thank you for your question. We are sorry to hear that this antibody did not perform as expected. It is very difficult to say which band is which without having more information. Please reach out to our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody and make sure you get the results you are looking for!

Respondida por: Tech Service

Fecha de publicación: 2017-06-07

Rated 2 de 5 por Ted Chen de OKI bought this few months ago, it did not perform really well in western blot

Fecha de publicación: 2018-09-28

Rated 5 de 5 por RahulK de Highly specific antibodyWe bought this antibody for use in western, IF and IHC and we are highly satisfied by its performance. WB- 1:1000, IF- 1:250, IHC- 1:200

Fecha de publicación: 2017-03-09

Rated 5 de 5 por qiliqiang de Worked well in WBI used this antibody in WB (dilution 1:500), the band is wonderful.

Fecha de publicación: 2016-11-01

Rated 5 de 5 por biosure de worked wellI used this antibody in WB (dilution 1:500), the band is wonderful.

Fecha de publicación: 2016-10-31

Rated 1 de 5 por Thomas_Gantert de Multiple unspecific bands across antibodyMultiple unspecific bands across antibody dilutions (1:100-10:1000) and independent of protein loading (20-80ug). No band at expected size. Mouse liver tissues were used.

Fecha de publicación: 2016-01-12

Rated 4 de 5 por Lin_Song de This has worked well in WBThis has worked well in WB.

Fecha de publicación: 2015-11-04

Rated 5 de 5 por NN de This has worked well in WBThis has worked well in WB

Fecha de publicación: 2015-10-27

Rated 4 de 5 por Dan de Used in IF and got good staining at 1:100 dilutionUsed in IF and got good staining at 1:100 dilution.

Fecha de publicación: 2015-09-24

PGC-1α Anticuerpo (H-300) Reseñas - página 2

PGC-1α Anticuerpo (H-300) clasificado 4.0 de 5 por 16.

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PGC-1α Anticuerpo (H-300): sc-13067

PGC1a Anticuerpo (D-5)

Paquete de PGC1a (D-5): m-IgG Fc BP-HRP

Paquete de PGC1a (D-5): m-IgGκ BP-HRP

Paquete de PGC1a (D-5): m-IgG2a BP-HRP

PGC1a Anticuerpo (D-5) AC

PGC1a Anticuerpo (D-5) HRP

PGC1a Anticuerpo (D-5) FITC

PGC1a Anticuerpo (D-5) PE

PGC1a Anticuerpo (D-5) Alexa Fluor® 488

PGC1a Anticuerpo (D-5) Alexa Fluor® 546

PGC1a Anticuerpo (D-5) Alexa Fluor® 594

PGC1a Anticuerpo (D-5) Alexa Fluor® 647

PGC1a Anticuerpo (D-5) Alexa Fluor® 680

PGC1a Anticuerpo (D-5) Alexa Fluor® 790

If the product is specified for mice, can it work on rat tissue&iquest;

what is the working concentration to be used

I am getting a band between 37 and 50 kDa with this antibody. Has someone else reported this?

What is the expected molecular weight for bands?

I performed an immunoprecipitation using this antibody (PGC-1 alpha Antibody - H-300) and I had a problem since the heavy chain (HC, 55 kDa) of the IP antibody were also recognized.

I used this antibody on cells and get 2 bands very close together which one is pgc1 alpha upper or lower

Paquete de PGC1a (D-5): m-IgG_2a BP-HRP

PGC1a Anticuerpo (D-5) Alexa Fluor^® 488

PGC1a Anticuerpo (D-5) Alexa Fluor^® 546

PGC1a Anticuerpo (D-5) Alexa Fluor^® 594

PGC1a Anticuerpo (D-5) Alexa Fluor^® 647

PGC1a Anticuerpo (D-5) Alexa Fluor^® 680

PGC1a Anticuerpo (D-5) Alexa Fluor^® 790

If the product is specified for mice, can it work on rat tissue¿