periplakin Antibody (AE11): sc-80605

Periplakin Antibody (AE11) is a mouse monoclonal IgG1 antibody that detects periplakin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Periplakin is a crucial member of the plakin family of proteins, primarily involved in the structural integrity of desmosomes and the epidermal cornified envelope, which are essential for maintaining cell adhesion and barrier function in epithelial tissues. Periplakin is localized to desmosomes, the interdesmosomal plasma membrane, and intermediate filaments, where anti-periplakin antibody (AE11) helps reveal its vital role in linking the cytoskeleton to cell junctions. Notably, the N-terminus of periplakin accumulates at the cell surface of microvilli, associating with cortical actin, which is important for cellular signaling and maintaining the architecture of the cell surface. The rod domain of periplakin is essential for the redistribution of envoplakin to desmosomes and the cytoskeleton through heterodimerization, while the linker domain is necessary for association with intermediate filaments. This intricate interplay of domains not only underscores the structural role of periplakin but also highlights periplakin′s importance in the assembly of the cornified envelope, a critical barrier in the skin that protects against environmental insults. Periplakin monoclonal antibody (AE11) serves as an invaluable tool for researchers studying the dynamics of cell adhesion and the pathophysiology of diseases such as paraneoplastic pemphigus, where disruptions in these processes can lead to severe blistering and skin fragility.

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periplakin Antibody (AE11) References:

1. Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation.  |  Milligan, G., et al. 2004. Biochem Soc Trans. 32: 878-80. PMID: 15494039
2. Periplakin-dependent re-organisation of keratin cytoskeleton and loss of collective migration in keratin-8-downregulated epithelial sheets.  |  Long, HA., et al. 2006. J Cell Sci. 119: 5147-59. PMID: 17158917
3. Cytolinker cross-talk: periplakin N-terminus interacts with plectin to regulate keratin organisation and epithelial migration.  |  Boczonadi, V., et al. 2007. Exp Cell Res. 313: 3579-91. PMID: 17662978
4. Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes.  |  Boczonadi, V. and Määttä, A. 2012. FEBS Lett. 586: 3090-6. PMID: 22841549
5. Serum periplakin as a potential biomarker for urothelial carcinoma of the urinary bladder.  |  Matsumoto, K., et al. 2014. Asian Pac J Cancer Prev. 15: 9927-31. PMID: 25520130
6. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers, and Cornified Envelope Precursor Proteins.  |  Boczonadi, V. and Määttä, A. 2016. Methods Enzymol. 569: 309-29. PMID: 26778565
7. SUMOylation of periplakin is critical for efficient reorganization of keratin filament network.  |  Gujrati, M., et al. 2019. Mol Biol Cell. 30: 357-369. PMID: 30516430
8. Desmoplakin and periplakin genetically and functionally contribute to eosinophilic esophagitis.  |  Shoda, T., et al. 2021. Nat Commun. 12: 6795. PMID: 34815391
9. CD103 and periplakin are potential biomarkers for response of metastatic melanoma to pembrolizumab.  |  Edmonds, NL., et al. 2022. Melanoma Res. 32: 440-450. PMID: 36169985
10. Targeting Periplakin of Novel Benzenesulfonamides as Highly Selective Agonists for the Treatment of Vitiligo.  |  Zhong, H., et al. 2024. J Med Chem. 67: 19323-19341. PMID: 39485487

The preferred periplakin antibody is periplakin Antibody (G-1): sc-365530.