Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PEPT1 CRISPR/Cas9 KO Plasmid (h): sc-401259
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PEPT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PEPT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PEPT1 Antibody (E-3): sc-373742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PEPT1 CRISPR/Cas9 KO Plasmid (h) | sc-401259 | 20 µg | $397.00 |
Overview
SLC15A1 encodes PEPT1, an apical proton-coupled oligopeptide transporter that mediates uptake of dietary di- and tripeptides and a broad range of peptide-like substrates across epithelial barriers. PEPT1 activity integrates transmembrane H+ gradients with nutrient absorption, linking intestinal transport to cellular amino acid availability and downstream metabolic and stress-response processes. Expression is highest in small intestine and can be induced in other epithelia under inflammatory or metabolic perturbation, influencing substrate flux in barrier tissues. Dysregulated SLC15A1/PEPT1 function has been investigated in contexts including intestinal inflammation, malabsorption phenotypes, and altered handling of peptide-mimetic compounds relevant to pharmacology and toxicology research.
PEPT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC15A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC15A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC15A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PEPT1 protein expression.
This CRISPR knockout system enables efficient generation of SLC15A1-deficient cell models for investigation of PEPT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC15A1 exon(s) critical for PEPT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC15A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PEPT1 CRISPR/Cas9 KO Plasmid (h) and PEPT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC15A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PEPT1 HDR Plasmid (h) and PEPT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC15A1 homology arms to support homology-directed repair at defined SLC15A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.