PEA-15 Antibody (H-3): sc-166678

PEA-15 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PEA-15 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PEA-15 (H-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PEA-15 protein, also known as Phosphoprotein Enriched in Astrocytes, plays a crucial role in regulating apoptotic signaling pathways due to a unique structure, which includes a death effector domain (DED) within its first 80 amino acids. This DED is essential for PEA-15′s ability to inhibit Ras suppression, thereby influencing cell survival and apoptosis. PEA-15 exists in three forms: a non-phosphorylated form and two phosphorylated forms, which are regulated by protein kinase C (PKC). The phosphorylation status of PEA-15 changes in response to microtubule dynamics, indicating colocalization with these structures. While predominantly expressed in the central nervous system, PEA-15 is also found in lower levels in the liver and kidney, and at higher levels in muscle tissue. PEA-15 is also referred to as Phosphoprotein Enriched in Diabetes (PED) due to increased expression in individuals with type 2 diabetes, highlighting a potential role in metabolic regulation and disease.

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PEA-15 Antibody (H-3) References:

1. PEA-15 modulates TNFalpha intracellular signaling in astrocytes.  |  Sharif, A., et al. 2003. Ann N Y Acad Sci. 1010: 43-50. PMID: 15033692
2. The expression of PEA-15 (phosphoprotein enriched in astrocytes of 15 kDa) defines subpopulations of astrocytes and neurons throughout the adult mouse brain.  |  Sharif, A., et al. 2004. Neuroscience. 126: 263-75. PMID: 15207344
3. PEA-15 is inhibited by adenovirus E1A and plays a role in ERK nuclear export and Ras-induced senescence.  |  Gaumont-Leclerc, MF., et al. 2004. J Biol Chem. 279: 46802-9. PMID: 15331596
4. Targetting PED/PEA-15 for diabetes treatment.  |  Fiory, F., et al. 2017. Expert Opin Ther Targets. 21: 571-581. PMID: 28395542
5. PEA‑15 contributes to the clinicopathology and AKT‑regulated cisplatin resistance in gastric cancer.  |  Jiang, X., et al. 2019. Oncol Rep. 41: 1949-1959. PMID: 30569123
6. PEA-15 engages in allosteric interactions using a common scaffold in a phosphorylation-dependent manner.  |  Ikedife, J., et al. 2022. Sci Rep. 12: 116. PMID: 34997083
7. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.  |  Chinnaiyan, AM., et al. 1995. Cell. 81: 505-12. PMID: 7538907
8. Cellular expression, developmental regulation, and phylogenic conservation of PEA-15, the astrocytic major phosphoprotein and protein kinase C substrate.  |  Danziger, N., et al. 1995. J Neurochem. 64: 1016-25. PMID: 7861130
9. Characterization of PEA-15, a major substrate for protein kinase C in astrocytes.  |  Araujo, H., et al. 1993. J Biol Chem. 268: 5911-20. PMID: 8449955
10. The major astrocytic phosphoprotein PEA-15 is encoded by two mRNAs conserved on their full length in mouse and human.  |  Estellés, A., et al. 1996. J Biol Chem. 271: 14800-6. PMID: 8662970
11. PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus.  |  Condorelli, G., et al. 1998. EMBO J. 17: 3858-66. PMID: 9670003
12. The death effector domain of PEA-15 is involved in its regulation of integrin activation.  |  Ramos, JW., et al. 1998. J Biol Chem. 273: 33897-900. PMID: 9852038

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PEA-15 Antibody (H-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-PEA-15 Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PEA-15 Antibody (H-3) Specifications

Alternate Product Name: PEA-15

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-130

Epitope Target: PEA-15

Epitope Species: human

Product Reactivity/Detection: PEA-15

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for PEA-15 Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin