PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h): sc-400107-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h) and PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PDGFRA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDGF Receptor alpha/PDGFRA Antibody (C-9): sc-398206
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h) | sc-400107-NIC | 20 µg | $410.00 | |||
PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h2) | sc-400107-NIC-2 | 20 µg | $410.00 |
PDGFRA encodes platelet-derived growth factor receptor alpha (PDGFRα), a receptor tyrosine kinase that binds PDGF ligands to initiate dimerization and autophosphorylation. Activated PDGFRα engages downstream signaling networks including PI3K–AKT, RAS–MAPK, and PLCγ pathways, coordinating proliferation, survival, migration, and mesenchymal lineage differentiation. PDGFRA activity contributes to stromal–epithelial crosstalk and extracellular matrix remodeling during development and tissue homeostasis. Dysregulated PDGFRA signaling and structural alterations are implicated in oncogenic growth programs and fibrotic remodeling, making it a frequent target in studies of receptor tyrosine kinase biology and tumor microenvironment signaling.
PDGF Receptor alpha/PDGFRA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PDGFRA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PDGFRA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PDGFRA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PDGFRA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.