Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pax-7 CRISPR/Cas9 KO Plasmid (m): sc-422120
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pax-7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pax-7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pax-7 Antibody (PAX7): sc-81648
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-7 CRISPR/Cas9 KO Plasmid (m) | sc-422120 | 20 µg | $397.00 |
Overview
Pax7 encodes Pax-7, a paired box transcription factor that defines and maintains skeletal muscle satellite cells and coordinates myogenic lineage commitment during development and regeneration. Pax-7 regulates gene expression programs controlling progenitor self-renewal, survival, and differentiation, and intersects with signaling networks such as Notch and Wnt that tune stem cell quiescence and activation. In mouse models, altered Pax-7 function perturbs muscle repair dynamics and satellite cell pool homeostasis, supporting its relevance for studying mechanisms underlying muscle wasting, impaired regeneration, and developmental myopathies. Pax-7 is also used as a marker and regulator in neuromuscular biology and cell fate studies where transcriptional control of myogenesis is a key endpoint.
Pax-7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pax7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pax7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pax7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pax-7 protein expression.
This CRISPR knockout system enables efficient generation of Pax7-deficient cell models for investigation of Pax-7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pax7 exon(s) critical for Pax-7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pax7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pax-7 CRISPR/Cas9 KO Plasmid (m) and Pax-7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pax7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pax-7 HDR Plasmid (m) and Pax-7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pax7 homology arms to support homology-directed repair at defined Pax7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.