Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pax-6 CRISPR/Cas9 KO Plasmid (m): sc-422119
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pax-6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pax-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pax-6 Antibody (PAX6): sc-81649
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-6 CRISPR/Cas9 KO Plasmid (m) | sc-422119 | 20 µg | $397.00 |
Overview
Mouse Pax6 encodes the paired box transcription factor Pax-6, a master regulator of ocular and central nervous system development that controls lineage specification, neurogenesis, and tissue patterning. Pax-6 binds enhancer elements to coordinate gene expression programs governing progenitor proliferation and differentiation, integrating with developmental signaling networks including Wnt, SHH, and FGF pathways. In mature tissues, Pax-6 contributes to maintenance of neuronal identity and pancreatic endocrine gene regulation, supporting glucose homeostasis. Dysregulated Pax6 activity or dosage is strongly linked to congenital eye malformations and neurodevelopmental phenotypes, making it a key target for mechanistic studies of developmental gene regulatory circuits.
Pax-6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pax6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pax6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pax6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pax-6 protein expression.
This CRISPR knockout system enables efficient generation of Pax6-deficient cell models for investigation of Pax-6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pax6 exon(s) critical for Pax-6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pax6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pax-6 CRISPR/Cas9 KO Plasmid (m) and Pax-6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pax6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pax-6 HDR Plasmid (m) and Pax-6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pax6 homology arms to support homology-directed repair at defined Pax6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.